Product Details

Creative Biogene offers a diverse range of expression vectors tailored to meet the needs of researchers in molecular biology and genetic engineering. Our collection includes specialized host vectors designed for cloning and expression of genes from different species. These vectors feature various engineered selection markers, antibiotic resistance, localized expression capabilities, and cloning site sizes to ensure efficient gene expression and protein production. Additionally, our vectors are compatible with downstream applications such as PCR, gene transduction, and gene transfection, offering versatility and flexibility in experimental design.

Key Features of Our Expression Vector

Versatility: Our expression vectors are suitable for a wide range of applications, including cloning, gene expression, and protein production.

Customization: We offer customizable vector solutions to meet specific research needs, ensuring flexibility and adaptability.

Quality Assurance: All our vectors undergo rigorous quality control to ensure reliability and performance consistency.

Comprehensive Collection: With our extensive collection of expression vectors, researchers can easily find the vectors that best suit their experimental requirements.

Expert Support: Our team of experts is available to provide assistance and guidance in choosing the most appropriate vectors for specific research goals, ensuring successful outcomes.

Expression Vector List

Application

Expression vectors are indispensable tools in molecular biology and genetic engineering, facilitating the efficient expression of DNA inserts in various host systems. These vectors serve different purposes ranging from protein expression in bacterial, yeast, and mammalian systems to cloning and library construction. Designed with specialized features, expression vectors allow precise control over gene expression, ensuring optimal protein production. They are widely utilized in research fields including protein biochemistry, structural biology, drug discovery, and functional genomics. With a diverse range of expression vectors available, researchers can choose the appropriate vector system tailored to their specific experimental requirements, accelerating scientific discoveries and advancements in biotechnology. Creative Biogene's Expression Vector products can be employed in various experimental studies, including:

• Protein expression and purification experiments: Utilizing vectors such as pQE, pRSET, pGEX, and pMAL for protein expression and purification, aiming to obtain high-purity target proteins.
• Protein interaction studies: Conducting protein interaction experiments using vectors containing tags or fusion proteins, such as pGEX and pMAL, to investigate protein-protein interactions.
• Molecular cloning and genome library construction: Employing vectors with appropriate restriction enzyme cleavage sites for gene cloning and library construction, facilitating molecular biology research.
• Transgenic and gene editing experiments: Using vectors such as pMJ806, lentiCRISPR v2 and pGAPZ for gene transfection, transformation, or editing, enabling the construction of transgenic animal models or application of gene editing technologies.
• Drug screening and functional genomics research: Performing drug screening experiments or CRISPR/Cas9-mediated genome editing experiments using vectors, facilitating drug development and functional genomics research.

Case Study

Case Study 1

The vector facilitated in planta RNA interference targeting Mj2G02, leading to impaired nematode parasitism. Additionally, Mj2G02-transgenic Arabidopsis lines, created using the vector, exhibited increased susceptibility to M. javanica. Through an Agrobacterium-mediated transient expression system, the researchers demonstrated Mj2G02's localization in plant cell nuclei and its ability to suppress Gpa2/RBP-1-induced cell death. Furthermore, using plant immune response assays, RNA-Seq, quantitative reverse transcription PCR, and HPLC analysis, they revealed Mj2G02's interference with the host jasmonic acid (JA) signaling pathway, suggesting its role in facilitating nematode parasitism by suppressing the plant immune response.

Figure 1. The subcellular localization of Mj2G02 in plant cells was investigated by researchers using the pCAMBIA vector. Arabidopsis protoplasts and Nicotiana benthamiana leaves were transformed, and the expression of Mj2G02Δsp:eGFP was confirmed via Western blot analysis. Fluorescence microscopy revealed nuclear localization of Mj2G02Δsp:eGFP, while eGFP controls exhibited signals in both cytoplasm and nucleus. (Song H, et al., 2021)

Case Study 2

Several research studies have shown that β-amylase is induced in response to abiotic stress. Researchers aimed to investigate the specific induction of β-amylase genes (BMY7 and BMY8) in response to temperature extremes. Using a temperature response profile, they demonstrated that induction was specific to these two gene members, which encode proteins targeted to the chloroplast stroma. Through time course experiments, they correlated β-amylase induction with maltose accumulation, suggesting a potential role for these enzymes in stabilizing the chloroplast stroma under acute temperature stress. The study highlights the targeted use of vector technology to elucidate the specific response of key genes to environmental cues, providing insights into the molecular mechanisms underlying stress responses in plants.

Figure 2. In the in vitro compatible solute assay for three enzymes, SspI, G6PDH, and ADH were exposed to heat with or without different concentrations of maltose, Glc, Suc, and trehalose. The protective effects of soluble sugars were assessed using vector-based assays. In this assay, functional SspI enzyme cleaves the pGEX-4T-2 plasmid twice (3.77 kb and 1.19 kb bands), while heat-damaged SspI cuts it once (5-kb open circle). (Kaplan F, et al., 2004)

Case Study 3

While the long noncoding RNA UCA1 is known to be upregulated in various cancers, including pancreatic ductal adenocarcinoma (PDAC), its specific functional roles in PDAC have yet to be fully understood. In this study, researchers aimed to investigate the functional roles of the long noncoding RNA UCA1 in pancreatic ductal adenocarcinoma (PDAC). They utilized the pSL-MS2-12X system to explore the mechanisms underlying UCA1's effects on PDAC progression. The results demonstrated that UCA1 significantly promoted cell proliferation, tumor growth, and stemness maintenance in PDAC cell lines, both in vitro and in vivo. Furthermore, they elucidated the molecular mechanisms by which UCA1 upregulated oncogenic KRAS activity and expression through interactions with hnRNPA2B1 and miR-590-3p. This reciprocal regulation between UCA1 and KRAS suggests the existence of a crucial UCA1-KRAS axis in PDAC progression. These findings highlight the potential of targeting UCA1 for the development of novel therapies for PDAC.

Figure 3. The effect of UCA1 on KRAS expression was investigated by researchers using pSL-MS2-12X. Changes in KRAS levels were observed in PaTu8988 and PANC-1 cells transfected with pSL-MS2-12X, indicating its role in modulating KRAS expression. (Liu Y, et al., 2019)

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