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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | MOLM-13 cells are a human acute myeloid leukemia (AML) cell line. The cell line was established from the peripheral blood of a 20-year-old male with acute myeloid leukemia, AML FAB M5a, who relapsed in 1995 after an initial case of myelodysplastic syndrome (MDS, refractory anemia with excess blasts, RAEB). These cells are widely used as an in vitro model of AML to study the mechanisms underlying the development of leukemia and to test the efficacy of potential therapeutic agents. MOLM13 cells exhibit many characteristics of AML cells, including the ability to rapidly proliferate in vitro and to form tumors when injected into immunocompromised mice. MOLM-13 cells have been used to study the role of various signaling pathways in AML, including the FLT3, Ras/MEK/ERK, and PI3K/Akt/mTOR pathways. These pathways are known to be dysregulated in a variety of cancers, including AML, and targeting them with therapeutic agents has shown promise in preclinical studies. |
| Tissue | Peripheral blood |
| Disease | Acute Myeloid Leukemia |
| Morphology | Most cells are round growing in suspension |
| Gender | Male |
| Age | 20 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug testing and development 3. Genetic studies 4. Signal transduction pathway studies 5. Immunological studies 6. Genomic and proteomic analysis 7. Resistance mechanism research |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | MOLM-13 cells have also been used to test the efficacy of various chemotherapeutic drugs, including cytarabine and daunorubicin. In addition, these cells have been used to develop and test novel targeted therapies, such as FLT3 inhibitors. The MOLM-13 CDX mouse model is a leukemia model used to explore the interaction between treatment efficacy and cancer progression while maintaining the tumor microenvironment. |
| Characteristics | |
| Karyotype | Human hyperdiploid karyotype with 4% polyploidy |
| Immunology | CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR -; |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | Seed at approximately 1.0 x 106 cells/mL; split saturated cultures 1:2 to 1:3 every 2-3 days; maintain at approximately 0.4-2.0 x 106 cells/mL; viability may drop below 50% after thawing, but cells recover after 4-5 days (recommended to start with 12-well or 24-well plates) |
| Subcultivation Ratio | The ratio of 1:2 to 1:3 is recommended. |
| Medium Renewal | Every 2 to 3 days |
| Culture Medium | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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