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MicroRNAs (miRNAs) are 18–24 nt RNA molecules that regulate the stability or translational efficiency of target mRNAs. Diverse functions have been attributed to miRNAs including the regulation of cellular proliferation, differentiation, and apoptosis. Moreover, significant evidence has accumulated implicating an important role for miRNAs in the development of cancer. Introduction miRNAs are initially transcribed as long precursor transcripts known as primary microRNAs (pri-miRNAs). Within these transcripts, the mature miRNA sequences are found in ~60-80 nt hairpin structures. The double-stranded RNA structure of the hairpins in a pri-miRNA is recognized by a nuclear protein DGCR8. DGCR8 associates with the enzyme Drosha to release hairpins from pri-miRNAs by cleaving RNA about 11 nt from the hairpin base and the resulting product is called a precursor-miRNA (pre-miRNA). In cytoplasm, the pre-miRNA hairpin is cleaved by the RNase III enzyme Dicer to generate an imperfect miRNA:miRNA duplex about 22 nucleotides in length. One strand of the mature miRNA is usually incorporated into the RNA-induced silencing complex (RISC) where the miRNA and its mRNA target interacts and leads to translational repression or target degradation.
Recombinant adenoviruses have enormous potential in both research and therapeutic applications. There are many advantages they provide when introducing genetic material into host cells. The virus has been used to infect numerous mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines that have low transfection efficiency with liposome. After entering cells, the virus remains epichromosomal (i.e. does not integrate into the host chromosome and thus does not activate or inactivate host genes). Many studies are done using adenovirus to deliver either the pre-microRNA or mature microRNA into the targeted cells for translational repression. The adenovirus carrier is an outstanding tool for in vivo delivery of microRNA compared with other methods.
As the leader in adenovirus manufacturing, Creative Biogene is proud to offer adenovirus microRNAs (miRNAs) with comprehensive coverage of the human genome. Our miRNAs are delivered to you packaged in adenovirus and are ready to use for efficient gene silencing. If you don’t have time to make your own miRNA expression adenovirus, there is a large number of hsa-miRNAs successfully integrated into premade adenovirus at Creative Biogene, which are ready-to-transfect. Besides, we also are offering custom miRNA adenovirus construction and production services to fit your research budgets.