Colon-26 Cell Line

Brief Introdution
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	The Colon-26 cell line is derived from mouse adenocarcinoma and was established after colon cancer induction in BALB/c female mice using N-Nitroso-N-methylurethane (NMU). This particular carcinogen is administered rectally, a method that effectively mimics the development of colorectal cancer. In 1975, Corbett et al. first reported the establishment of the Colon-26 cell line, which marked a significant progress in the study of carcinogen-induced cancer in animal models. Colon-26 cells transplant and maintain adenocarcinoma characteristics of the original tumor, making them a valuable tool in oncology research, particularly as it relates to colorectal cancer. This cell line is particularly useful for examining the efficacy of anticancer therapies and the molecular pathways involved in colorectal cancer progression.
Disease	Carcinoma
Morphology	Epithelial-like
Age	6 months
Gender	Female
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer research 2. Study immune system interactions 3. Vaccine development 4. Study the effects of immune checkpoint inhibitors 5. Gene expression and molecular pathway studies 6. Drug screening and development 7. In vivo models
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	Mice with C26 carcinoma (also known as colon 26 carcinoma and adenocarcinoma) represent a well-characterized and widely used model of cancer cachexia. Growth of C26 tumors results in loss of body and muscle weight, primarily through enhanced fat and protein catabolism. In general, a 10% reduction in tumor weight relative to total body weight results in a 20-25% reduction in skeletal muscle weight and greater fat consumption. As tumors grow, hepatomegaly and splenomegaly are also observed, as well as activation of the acute phase response and increased levels of pro-inflammatory cytokines.
Characteristics
Tumorigenic	In Balb/c mice
Viruses	MAP-test negative: Sendai, Ektromelie, K-Virus, Kilham, Reo 3, PVM, Polyoma, LCM, M.pulmonis, MVM, MHV, LDV, RCV/SDA, M-Adenovirus, Theiler's GD VII, Toolan's H-1, B.piliformis
Culture Conditions and Handling
Subculturing	1. Remove the old medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, cells were gently mixed with 10 ml of medium to resuspend and centrifuged at 300xg for 3 min. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A ratio of 1:4 to 1:6 is recommended.
Culture Medium	RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%;Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RR0452	GFP Stable Cell Line - Colon26	Inquiry
CSC-RR0461	Luciferase Stable Cell Line-Colon26	Inquiry
CSC-RR0462	GFP/Luc Reporter Cell Line - Colon 26	Inquiry

* For research use only. Not intended for any clinical use.

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