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The Tet-Off/Tet-On system is a well-established and widely used inducible gene expression system in eukaryotic cells. It utilizes regulatory elements derived from the tetracycline operon in E. coli and has been adapted for efficient control of exogenous gene expression in various eukaryotic organisms, including cells and transgenic mice. This system offers several advantages, including quantitative and specific control over gene expression, high efficiency, non-toxicity, and tight regulation of on/off states.
The Tet-Off system allows for gene expression to be turned off in the presence of tetracycline or its derivative, doxycycline. In the absence of the inducer, the Tet regulatory protein (tTA) binds to the Tet-responsive promoter (TRE), preventing the expression of the gene of interest (GOI). However, upon addition of tetracycline or doxycycline, the inducer binds to tTA, causing a conformational change that releases tTA from TRE, enabling the expression of the GOI. Conversely, the Tet-On system allows for gene expression to be turned on in the presence of tetracycline or doxycycline. In the absence of the inducer, the reverse Tet regulatory protein (rtTA) binds to the TRE, preventing the expression of the GOI. When tetracycline or doxycycline is added, it binds to rtTA, inducing a conformational change that enables rtTA to bind to TRE and initiate the expression of the GOI.
At Creative Biogene, we specialize in the development of inducible expression stable cell lines, providing you with a powerful tool for precise control over gene expression in your research or biotechnological applications. Our experienced team of scientists offers comprehensive services to support the construction of inducible cell lines tailored to your specific needs. If you are interested in our inducible expression stable cell line development services or have any specific requirements, please feel free to contact us. Throughout the process, we maintain stringent quality control measures to ensure the reliability, reproducibility, and consistency of our inducible expression stable cell lines. Our aim is to empower your research with a versatile tool for investigating gene function, drug screening, or other applications requiring precise control over gene expression.
Figure 1. Flow cytometry analysis of induced GFP expression in HEK293-Tet/on-GFP clones.
Figure 2. Cell images of HEK293-Tet/on-GFP clones.
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