Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Rattus norvegicus, rat |
| Cell Line Description | The GH3 cell line is a rat pituitary tumor cell clone isolated in 1965 from MtT/W5 prolactinomas in female Wistar-Furth rats. It is a well-known model in endocrinology due to its dual ability to synthesize and secrete both growth hormone (GH) and prolactin (PRL). GH3 cells are highly sensitive to a variety of physiological stimuli, including thyrotropin-releasing hormone (TRH), which significantly enhances prolactin secretion and inhibits growth hormone release. Because of these endocrine functional properties, this cell line is widely used to study hormone regulation, signal transduction, and calcium-dependent exocytosis mechanisms. |
| Tissue | Pituitary gland |
| Disease | Tumor |
| Morphology | Epithelial |
| Gender | Female |
| Age | 7 months |
| Product Format | Frozen |
| Growth Mode | Adherent and small clusters in suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Research on the synthesis and secretion of growth hormone (GH) and prolactin (PRL) 2. Research on hormone signaling pathways (e.g., thyrotropin-releasing hormone [TRH], estrogen) 3. Research on ion channel function and calcium signaling in endocrine cells 4. Drug screening for endocrine-related diseases 5. Assessment of pituitary tumor cell proliferation and survival |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in syngeneic Wistar-Furth rats |
| Karyotype | Aneuploid; modal number varies among sublines |
| Hormone Secretion | Secrete Growth Hormone (GH) and Prolactin (PRL) |
| Receptors Expressed | TRH receptors, Estrogen receptors, Somatostatin receptors |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with Ca2+/Mg2+-free PBS or 0.25% trypsin-EDTA solution to remove residual serum. 3. Add trypsin-EDTA solution and observe until the cell layer disperses (usually 5 to 10 minutes). 4. Add complete culture medium and gently pipette to disperse cell clumps. 5. Aliquot the cell suspension into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended |
| Culture Medium | F-10 Ham's Medium or DMEM/F12 supplemented with 15% Horse Serum and 2.5% Fetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00764 | Luciferase Reporter Cell Line - GH3 | Inquiry |
| CSC-RR00765 | GFP Reporter Cell Line - GH3 | Inquiry |
Copyright © Creative Biogene. All rights reserved.