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Phagemid vectors are versatile tools derived from both coliphages and plasmids, featuring a plasmid replication origin for double-stranded DNA isolation and a phage replication origin for single-stranded replication. Creative Biogene offers a range of phagemid vectors for diverse research needs, including protein-protein interaction studies, enzyme inhibitor screening, and ligand screening. With customizable features and robust performance, Creative Biogene's phagemid vectors empower researchers to advance their molecular biology studies and accelerate scientific discoveries.
Key Features of Our Custom miRNA qPCR Primers Set
The General Phagemid Vector is a flexible tool for gene insertion and expression in phage display. Highly customizable, it suits gene cloning, protein expression, and purification.
Specifically for phage display, the pComb3H Set expresses proteins or peptides on phage particles, facilitating interaction screening and affinity determination.
The pComb3X Set offers flexibility and selectivity for phage display, enabling the screening of proteins with specific binding affinities.
The Original pCombs, derived from classical vectors, are widely used in gene cloning, protein engineering, and antibody screening, providing stable expression and reliable results for various experiments.
Phagemid vectors are versatile tools in molecular biology, facilitating protein display, interaction studies, and antibody library construction. These hybrids combine plasmid and phage characteristics, enabling efficient replication in Escherichia coli and protein display on M13 phages. Featuring surface protein genes and selectable markers, they support diverse applications in genetic, protein, and antibody engineering, drug screening, and vaccine development. By cloning genes of interest and expressing them in bacterial hosts or displaying them on phages, researchers can swiftly analyze and screen target proteins, advancing research in various fields:
Case Study 1
Despite the initial incorporation of noncanonical amino acids (ncAAs) into phage libraries through amber suppression almost twenty years ago, their utilization in drug discovery has been constrained by inherent biases favoring sense-containing phages. Researchers employed the pADL-pIII technique to overcome biases in phage libraries and enrich clones containing noncanonical amino acids (ncAAs). This innovation facilitated active site-directed ligand evolution of peptides, utilizing ncAAs as anchors to target the binding of peptides to the active site of a protein. Specifically, they developed phage-displayed peptide libraries containing genetically encoded butyryl lysine and selected ligands binding SIRT2. These ligands were further modified to yield potent inhibitors of SIRT2 with low nanomolar affinity, showcasing the efficacy of the pADL-pIII approach in drug discovery.
Figure 1. To produce phagemid particles, Top10 cells were transformed with three plasmids: (1) pADL(NNK)7gIII encoding the amber-obligate phagemid library, (2) pEDF-PhdRS containing genes encoding PhdRS and tRNAPyl, and (3) M13KO7(pIII−). When transformed cells were grown in the presence of one of the ncAAs 1–4, a 36- to 83-fold increase in phagemid particle production was observed compared with when no ncAA was present. (Tharp JM, et al., 2020)
Case Study 2
The accidental finding of heavy-chain antibodies in camelids and the development of VHHs have become valuable tools for research and potential therapeutics. Researchers utilized pCDisplay to express and characterize the single-domain 15-kDa antibody SBT-100 (anti-STAT3 B VHH13) against human STAT3. The purpose was to investigate its selective binding to STAT3 and its inhibitory effects on phosphorylated STAT3 (p-STAT3) function. The study demonstrated that SBT-100 effectively suppressed cell proliferation in triple-negative breast cancer cells and inhibited tumor growth in xenograft models without toxicity. These findings suggest the potential therapeutic utility of SBT-100 in targeting STAT3-mediated signaling pathways in cancer.
Figure 2. The use of Phagemid Vector allowed for the expression and display of camelid antibodies (VHHs) by researchers for targeting STAT3 protein. This approach enabled efficient screening and selection of high-affinity VHHs, which could then be utilized for various downstream applications such as immunoprecipitation and Western blotting. (Singh S, et al., 2018)
Q: Do Creative Biogene's Phagemid Vector products possess high stability to ensure the reliability of my experimental results?
A: Yes, our Phagemid Vector products are carefully designed and rigorously validated for high stability and reliability. We employ high-quality materials and advanced manufacturing processes to ensure that each product meets high standards, thereby ensuring the accuracy and reproducibility of your experimental results.
Q: Are Creative Biogene's Phagemid Vector products suitable for my specific experimental purposes? How do I choose the most suitable vector for my needs?
A: Our Phagemid Vector products cover various experimental requirements, including gene expression, protein display, antibody library construction, etc. Our professional team can provide customized recommendations based on your experimental objectives and sample characteristics to help you choose the most suitable vector.
Q: I am very concerned about the performance and stability of Creative Biogene's Phagemid Vector products. Is there a related quality control and quality assurance system?
A: Yes, our company strictly adheres to the ISO 9001 quality management system, and all products undergo rigorous quality control and quality assurance procedures. We are committed to providing customers with high-quality, stable, and reliable Phagemid Vector products to meet their research needs.
Q: I am not very familiar with the usage and operation procedures of Phagemid Vector products. Is there relevant technical support and customer service available?
A: Our customer service team consists of experienced professionals who can provide detailed product information, operating instructions, and technical support. If you encounter any problems or need assistance during use, you can contact us at any time, and we will be happy to provide support and assistance.
| Cat.No. | Product Name | Price |
|---|---|---|
| VPT4010 | pComb3 vector | Inquiry |
| VPT4011 | pComb8 vector | Inquiry |
| VPT4012 | pComb3X vector | Inquiry |
| VPT4013 | pComb3XSS vector | Inquiry |
| VPT4014 | pComb3XTT vector | Inquiry |
| VPT4015 | pComb3XLambda vector | Inquiry |
| VPT4016 | pComb3H vector | Inquiry |
| VPT4017 | pComb3HSS vector | Inquiry |
| VPT4018 | pComb3HTT vector | Inquiry |
| VPT4019 | Pjf3h vector | Inquiry |
| VPT4020 | pIgG vector | Inquiry |
| VPT4021 | pCDisplay-2 vector | Inquiry |
| VPT4022 | pCDisplay-3M vector | Inquiry |
| VPT4023 | pCDisplay-3 vector | Inquiry |
| VPT4024 | pCDisplay-4 vector | Inquiry |
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