Dual Luciferase Assay Kit

Firefly luciferase has been used as a sensitive reporter to study a wide range of biological responses. However, the change of the expression of Firefly luciferase reporter can be due to a global effect instead of a specific effect. The accuracy of Firefly luciferase reporter can be improved by normalizing to a control reporter, such as Renilla luciferase reporter, in the same sample.

Firefly and Renilla luciferases have been widely used as co-reporters because they have distinct evolutionary origins and very different enzyme structures and substrates. Creative Biogene's dual luciferase assay kit is the perfect choice for high sensitivity detection of Firefly luciferase (FLuc) and Renilla luciferase (Rluc) activities. The Firefly luciferase luminescence is produced by one reagent, while a second reagent simultaneously quenches the Firefly luciferase and produces Renilla luciferase luminescence. It works great in many different eukaryotic cell types using micro-plate or single-tube luminometers. The dual luciferase assay kit is ideal for many applications, such as measuring the inhibitory effect of a miRNA on a target sequence, enhancer/promoter analysis, enzymatic screening, and transgene expression analysis.

The dual Luciferase reporter vectors are designed for using with the dual luciferase assay kit.

Features

1. High sensitivity — Reagents are optimized for greatest sensitivity of Firefly and Renilla luciferase signals.
2. Versatility — Designed for assays with many different eukaryotic cell types using micro-plates or single-tube luminometers.
3. Low background — Very low background luminescence. No subtraction is required from readings.
4. Simplicity — Renilla luciferase buffer and substrate contains the quencher for Firefly luciferase activity, enabling quick Glow and Stop-N-Glow two-step assays.
5. Stable – The luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time.
6. High-throughput – Simple homogeneous protocol minimizes handling steps to support high-throughput screening applications.

Applications

1. miRNA target identification and functional validation of predicted targets, or to study the regulatory effect of miRNAs on target genes.
2. Enhancer/promoter analysis.
3. Enzymatic screening.
4. Transgene expression analysis.