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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | K7M2-WT is a murine osteosarcoma cell line derived from the K7M2 cell line. The K7M2 cell line was established by screening for highly metastatic K7 cell lines in BALB/c mice. "WT" typically refers to the "wild-type" parental version of this highly aggressive clone. K7M2-WT is characterized by its fibroblast and osteoblast morphology, and its remarkable ability to spontaneously metastasize to the lungs after paratibial or subcutaneous injection. K7M2-WT is the preferred homology model for studying osteosarcoma biology, particularly the molecular drivers of lung metastasis, and for evaluating novel immunotherapies and chemotherapeutic agents. |
| Tissue | Bone |
| Disease | Osteosarcoma |
| Morphology | Fibroblastic; Osteoblastic |
| Gender | Not specified (derived from BALB/c mouse) |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Research on the mechanism of osteosarcoma lung metastasis 2. Construction of orthopedic tumor homologous animal models 3. Testing of novel immunotherapies and targeted drugs 4. Research on tumor-bone microenvironment interactions 5. Evaluation of surgical and radiotherapy-assisted models |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in BALB/c mice |
| Metastatic Potential | Extremely high spontaneous pulmonary metastasis rate |
| Phenotype | Maintains osteoblastic features including alkaline phosphatase activity |
| Genetic Profile | Characteristic of high-grade osteosarcoma with complex genomic instability |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca2+/Mg2+-free PBS buffer to remove all serum residue. 3. Add 2.0 mL of 0.25% trypsin-0.53 mM EDTA solution and incubate at 37°C for 3 to 7 minutes until cells detach. 4. Add complete culture medium to neutralize the trypsin and gently pipette to disperse cell clumps. 5. Aliquot the cell suspension into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Medium | DMEM or RPMI 1640 supplemented with 10% Fetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00786 | Luciferase Reporter Cell Line - K7M2-WT | Inquiry |
| CSC-RR01021 | GFP/Luc Reporter Cell Line - K7M2-WT | Inquiry |
| CSC-RR01200 | GFP Reporter Cell Line - K7M2-WT | Inquiry |
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