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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | DU145 (DU-145) is a human prostate cancer cell line. DU145, PC3, and LNCaP are considered standard prostate cancer cell lines used in therapeutic studies. The DU145 cell line was derived from central nervous system metastases from primary prostate adenocarcinoma that were excised during a parieto-occipital craniotomy in a 69-year-old white man. DU145 is not hormone sensitive and does not express prostate-specific antigen (PSA). DU145 cells had intermediate metastatic potential compared to PC3 cells, which had high metastatic potential. |
| Tissue | Prostate |
| Disease | Carcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 69 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 3D cell culture Studying prostate cancer biology Drug screening and development Biomarker discovery |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The DU145 cell line is not sensitive to hormones, is only weakly positive for acid phosphatase, and isolated cells form colonies in soft agar. These cells do not express prostate antigen. Ultrastructural analysis of cell lines and original tumors revealed microvilli, tonofilaments, desmosomes, any mitochondria, a well-developed Golgi apparatus, and heterogeneous lysosomes. |
| Characteristics | |
| Karyotype | This is a hypotriploid human cell line. Among the 30 metaphase counts, chromosomes 61 and 62 had the highest occurrence rate, with a high ploidy rate of 3%. t(11q12q), del(11)(q23), 16q+, del(9)(p11), del(1)(p32) and 6 other marker chromosomes were found in most cells. N13 is usually absent. The Y chromosome is abnormal due to translocation to an unknown chromosomal segment. The X chromosome exists as a single copy. |
| Tumorigenic | Yes, forms tumors in nude mice. |
| Metastatic | Brain |
| Antigen Expression | Blood Type O; Rh+ |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate cultures at 37℃. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:4 to 1:6 is recommended. |
| Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA. To prepare complete growth medium, add the following ingredients to basal medium: fetal bovine serum to a final concentration of 10%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
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