HOS Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	HOS (Human Osteosarcoma Cell Line) is an immortalized human cell line isolated in 1971 from a primary osteosarcoma in a 13-year-old Caucasian female patient. This cell line was established to facilitate research into the biology of bone cancer and the mechanisms of viral transformation. HOS cells exhibit a mixed morphology, comprising both fibroblast-like and epithelial-like cells. Their distinctive characteristics include rapid growth and relative ease of transfection. Although the parental HOS cell line demonstrates extremely low tumorigenicity in nude mice, chemical induction or viral transformation has yielded various highly tumorigenic derivative cell lines (e.g., KHOS and MNNG-HOS). Consequently, this cell line serves as a foundational model for investigating bone tumor progression, cell cycle regulation, and the molecular mechanisms underlying mesenchymal cell transformation.
Tissue	Bone
Disease	Osteosarcoma
Morphology	Mixed (Fibroblastic and Epithelial-like)
Gender	Female
Age	13 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Osteosarcoma biology and bone cancer progression research 2. Study of viral and chemical transformation mechanisms 3. Transfection studies and gene expression analysis 4. Drug screening and cytotoxicity assays for bone tumors 5. Investigation of mesenchymal cell differentiation and signaling
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Karyotype	Aneuploid; modal number in the triploid range; multiple chromosomal rearrangements
Tumorigenic	Low to No (Parental line); highly tumorigenic derivatives exist
Genetic Profile	TP53 mutation; RB1 mutation; flat-cell morphology
Expression Markers	Positive for alkaline phosphatase (low levels) and osteonectin
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:4 to 1:10 is recommended
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% to 10% (v/v) DMSO

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Cat.No.	Product Name	Price
CSC-RR00782	Luciferase Reporter Cell Line - HOS	Inquiry
CSC-RR01195	Nluc Reporter Cell Line - HOS	Inquiry

* For research use only. Not intended for any clinical use.

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