Gene Editing Cell Line Generation Service

CRISPR as a highly efficient gene editing tool can manipulate the genome of a cell to achieve gene knockout, knock-in, targeted gene mutation in specific cells. Gene-edited cell lines generated by CRISPR/Cas9 technology can be widely used for gene function research, signaling, recombinant protein preparation, drug discovery, and disease mechanism of action research. Creative Biogene's scientists utilize advanced CRISPR technology to efficiently and precisely modify specific genes in a variety of cell types, including but not limited to mammalian cells, stem cells, and other cells, based on the research needs of our clients. We are committed to providing comprehensive and efficient gene editing cell line generation services to accelerate your research.

Gene Editing Cell Line Generation Service at Creative Biogene

Our project specialists are experienced in creating a variety of stable gene-edited cell lines and can customize genome editing to your specific requirements. Below are the various types of editing services we offer utilizing CRISPR technology.

Service Type	Service Detail	Delivery Time
Knockout cell lines	Single gene knockout cell lines Multi gene knockout cell lines Large fragment excision cell lines	9-15 weeks
Conditional knockout cell lines	Combining the Cre-LoxP recombination system with CRISPR/Cas9 technology provides customers with stable conditional knockout cell lines.	9-15 weeks
Knock-in cell lines	We use the CRISPR/Cas9 system to establish efficient knock-in protocols for exogenous genes or fragments in different cells according to our clients' research demands.	12-25 weeks
Targeted mutant cell lines	Achieve pointwise mutation of the target gene base sequence, including deletion, insertion and substitution of all individual bases, using CRISPR/Cas9 technology.	12-25 weeks

Process of Gene Editing Cell Line Generation

Our project experts will work with you to develop customized gene-edited cell line generation strategies and provide one-stop cell line generation services. First, our experts will evaluate the cell lines you are interested in. The aim of the target evaluation is to assess the feasibility of gene knockout or knock-in for the target gene, as well as to test the transfection efficiency and monoclonal growth ability of the target cells in preliminary experiments. The process involves designing multiple sgRNAs, constructing vectors, and selecting sgRNAs with high editing efficiency through experiments conducted on cells. The next step is to introduce CRISPR vectors or RNP into recipient cells through electro- or viral transfection. Monoclonal screening and validation are then carried out, which includes sequencing to confirm gene knockdown at the genetic level (the default method), as well as optional methods such as FACS and WB to validate knockdown at the protein level. Finally, stable cell lines that have undergone gene editing are provided, ensuring they are mycoplasma-tested and free from contamination.

Process of Gene Editing Cell Line Generation

Advantages of Our Services

One-stop service solution from sgRNA design and synthesis to cell line screening.
Efficient gene editing tool based on CRSIPR technology.
Real-time project follow-up to ensure delivery on schedule.

Creative Biogene's team of experts is ready to work closely with you. We provide guidance on experimental design, target selection, and optimization strategies to ensure that your gene editing experiments are on schedule and successful. Contact us to learn more about our services.

* For research use only. Not intended for any clinical use.

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