Creative Biogene is the leading biotechnology company offering in vitro profiling of your deubiquitinating enzyme (DUB), DUB substrate, and/or DUB activator or inhibitor. As an experienced pioneer in this field, Creative Biogene is dedicating to providing you the most comprehensive DUB assays with the most competitive price.
Creative Biogene can provide services that characterize your known DUB or confirm your putative DUB by testing it against the classic DUB substrates and inhibitors currently available. Our experienced scientists will work close with your team to profile the activity of your DUB with any or all of our available substrates and inhibitors. Meanwhile, Creative Biogene can also test your inhibitor, substrate, or activator against a wide range list of full-length DUBs available.
Ubiquitin modifications can be reversed by the reaction of enzymes collectively known as deubiquitinating enzymes (DUBs). Deubiquitination is performed by the action of DUBs, ubiquitin specific proteases that can reverse target-protein ubiquitination by editing or disassembling polyubiquitin chains. DUBs play an important role in several aspects of the ubiquitin-proteasome system, and mutations in genes expressing DUBs have been implicated in a number of diseases, including hereditary cancer and neurodegeneration.
There are five families of DUBs. Four are cysteine proteases, and one family is metalloproteases. DUBs specifically cleave ubiquitin from ubiquitin-conjugated protein substrates, ubiquitin precursors, ubiquitin adducts and polyubiquitin (Alan, et al. 1998).
Fig.1 DUBs involved in different steps of the ubiquitination process (Karen, et al. 2008)
Creative Biogene offers the easiest way to quickly identify novel DUB inhibitors, by screening your compounds library across any of the more than 40 individual DUB targets on our panel of enzymes, which represent the entire human DUB superfamily. Our service is available in a wide range of different formats, including single point analysis, IC50 determination and high throughput screening for larger numbers compounds.
Advantages of DUB profiling assays in Creative Biogene:
- The most comprehensive human DUB targets on our panel
- A broad range of substrates available to monitor activity of DUBs
- Flexible assay, from single concentrations to high throughput screening, or determine IC50 values by standard 8-point half-log concentration-effect curves (IC50)
- Screening any combination of compounds against DUB
- Fast turnaround time and high efficiency
With years of experience in DUB screening and profiling services, Creative Biogene is confidence in providing compound screening assay against your DUB target with the most excellent platforms. We offer availability of various panels of DUB for your inhibitor identification. Based on proper communication and updated project progress on time, Creative Biogene is dedicated to accelerating your drug discovery project with high-quality and short lead time.
If you have any special requirements in DUB screening and profiling services, please feel free to contact us at firstname.lastname@example.org or 1-631-626-9181. We are looking forward to working together with your attractive projects.
- Karen, H. V. and Keith, D. W. (2008) ‘Protein partners of deubiquitinating enzymes’. Biochem. J., 414, 161-175
- Alan, D. A. and David, P. (1998) ‘Deubiquitinating Enzymes:A New Class of Biological Regulators’, Critical Reviews in Biochemistry and Molecular Biology, 33(5), 337-352