U251 MG Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	U251 MG is a cell line derived from human glioblastoma multiforme, a highly aggressive malignant brain tumor. The U251 MG human cell line was derived from a 75-year-old brain cancer patient diagnosed with glioblastoma astrocytoma. Genetically, U251 MG cells harbor mutations and changes typical of high-grade astrocytomas, including mutations in the TP53 gene and loss of heterozygosity on chromosome 10, which contains the PTEN gene. U251 MG cells are known to possess many characteristics common to glioblastoma, including rapid growth, high invasiveness, and resistance to chemotherapy and radiotherapy. These characteristics make them valuable tools for studying glioblastoma biology and testing new treatments for the disease. Additionally, U251 MG has been used in numerous studies focusing on therapeutic approaches, including chemoresistance, radiation therapy outcomes, and evaluation of novel anticancer compounds.
Tissue	Brain
Disease	Astrocytoma
Morphology	Epithelial-like
Gender	Male
Age	75 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Breast cancer research 2. Endocrine research 3. Genetic studies 4. Signal transduction research 5. Radiation studies 6. Toxicology testing 7. Metastasis research
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	The U-251 MG cell line was used to create the CDX (Cell Line Derived Xenograft) U-251 MG xenograft mouse model. The U-251 MG xenograft model is a highly invasive preclinical model containing mu-p53 and mu-PTEN. Monotherapy in the U 251 CDX model exhibits delayed tumor growth after administration with a CDK inhibitor (e.g., milciclib) and disruption of established tumor blood vessels after administration with a tumor blood vessel disrupting agent (e.g., vadimezan).
Characteristics
Protein Expression	Expression of GFAP and vimentin
Tumorigenic	Yes, in nude mice.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove old medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, cells were gently mixed with 10 ml of medium to resuspend and centrifuged at 300 x g for 3 min. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	The ratio of 1:3 to 1:5 is recommended.
Culture Medium	EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

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Cat.No.	Product Name	Price
CSC-RO01227	Cas9 Stable Cell Line - U251MG	Inquiry
CSC-RR00719	Luciferase Reporter Cell Line - U251MG	Inquiry

* For research use only. Not intended for any clinical use.

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