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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SiHa is a well-characterized human epithelial cell line isolated in 1970 from a primary tissue sample of a 55-year-old Japanese woman with cervical squamous cell carcinoma. These cells exhibit typical epithelial morphology and grow in an adherent monolayer. Biologically significant, SiHa contains a low copy number (approximately 1 to 2 copies per cell) of integrated human papillomavirus type 16 (HPV-16) genome. The cell line expresses p53 and pRB proteins, key targets of HPV E6 and E7 oncoproteins. Electron microscopy confirmed the presence of typical desmosomes at cell junctions and abundant tonofilaments in the cytoplasm, reflecting its squamous cell origin. |
| Tissue | Uterus; Cervix |
| Disease | Cervical Squamous Cell Carcinoma |
| Morphology | Epithelial-like |
| Gender | Female |
| Age | 55 years |
| Ethnicity | Asian (Japanese) |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 2 (Contains HPV-16) |
| Applications | 1. Cervical Cancer Research: Serves as a primary model for studying the molecular mechanisms of HPV-mediated tumorigenesis and development. 2. HPV Pathogenesis Research: Used to investigate the integration, expression, and regulation of high-risk HPV-16 viral genes (E6/E7) in the human cellular environment. 3. Drug Screening and Development: Widely used to evaluate the efficacy of anticancer drugs, particularly those targeting HPV-related malignancies, and to study drug resistance mechanisms. 4. Vaccines and Diagnostic Testing: Used for the development of HPV vaccines and the validation of self-sampling diagnostic tools for cervical cancer screening. 5. In vivo Tumor Modeling: Ideal for establishing xenograft models in nude mice to assess tumor growth dynamics and the therapeutic potential of novel interventions. |
| Shipped In | Dry ice |
| Storage Temperature | Below −140°C or in liquid nitrogen vapor phase |
| Characteristics | |
| Karyotype | Hypertriploid; modal chromosome number is 71 (range 69–72), occurring in 24% of cells. Polyploidy rate is approximately 7.6%. |
| Tumorigenic | Yes, in nude mice; forms poorly differentiated epidermoid carcinoma. |
| Virology Status | HPV-16 positive (1-2 copies per cell). |
| Genes Expressed | p53+, pRB+; integrates partial HPV16 sequences. |
| Markers Expressed | Epithelial cell adhesion molecule (EpCAM) is present in ~92% of cells; strong cytokeratin expression; negative for vimentin. |
| Isoenzymes | AK-1: 1; ES-D: 2; G6PD: B; GLO-I: 2; Me-2: 1; PGM1: 1; PGM3: 1. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Gently rinse the cell layer with calcium- and magnesium-free PBS buffer to remove residual serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of 0.25% (w/v) trypsin-0.53 mM EDTA solution (for T75 culture flasks) and observe under an inverted microscope until the cell layer disperses (usually 5 to 15 minutes). For cells that are difficult to disperse, incubate at 37°C to promote dispersion. 4. Add an equal volume of complete culture medium to neutralize the trypsin. 5. Gently pipette the cells and transfer them to centrifuge tubes. 6. Centrifuge at 125 x g for 5–10 minutes. 7. Resuspend the cell pellet in fresh complete culture medium and aliquot into new culture containers. |
| Medium Renewal | 2 to 3 times per week. |
| Subcultivation Ratio | A split ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | EMEM (Eagle's Minimum Essential Medium) or MEM + 10% Fetal Bovine Serum (FBS) + 2 mM L-glutamine. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | 90% to 95% complete growth medium supplemented with 5% to 10% (v/v) DMSO. |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02641 | Cas9 Stable Cell Line - SiHa | Inquiry |
| CSC-RR00830 | Luciferase Reporter Cell Line - SIHA | Inquiry |
| CSC-RR00831 | GFP Reporter Cell Line - SIHA | Inquiry |
| CSC-RR00975 | GFP/Luc Reporter Cell Line - SIHA | Inquiry |
| CSC-RR01235 | Nluc Reporter Cell Line - SIHA | Inquiry |
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