Stable cell lines carrying a reporter gene (a tag or a fluorescent protein) cassette using CRISPR targeted genome editing...
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Stable cell lines carrying a reporter gene (a tag or a fluorescent protein) cassette using CRISPR targeted genome editing via the HDR pathway is a powerful tool for monitoring endogenous target gene expression or biological processes without disrupting the target gene in vitro.
Based on years of experience and in-depth investigation, scientists at Creative Biogene can target specific DNA sequences and induce a double stranded break, taking advantage of recombination to create synthetic genetic genome of a host. An expression cassette containing reporter and resistance genes was knocked into the safe-harbor site or any other sites in host cells. Gene insertion at a safe-harbor site allows stable gene expression without any adverse effects on the fitness of the engineered cells.
Reporter Genes Knock-In Cell Line Generation Service Case
1. Knock-in Red Fluorescent Protein (RFP) gene into HEK293 cell line at the Rosa26 safe harbour site.
2. PCR detection of the genomes of transfected cells.
3. Sequencing results of the Rosa26 locus in RFP-positive HEK293 cells
Sequencing results of the upstream region of Rosa26 site.