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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SK-N-BE(2) is a human neuroblastoma cell line established in 1972, derived from a bone marrow biopsy specimen of a 2-year-old male patient following multiple rounds of chemotherapy and radiotherapy. This cell line serves as a model for highly aggressive and poorly differentiated neuroblastoma. A defining characteristic of the SK-N-BE(2) cell line is the presence of MYCN gene amplification and loss-of-function mutations in the TP53 gene, making it a critical model for studying high-risk, refractory neuroblastoma. SK-N-BE(2) cells exhibit a neuroblast-like morphology; while they typically grow as adherent monolayers, they are also capable of forming dense cellular aggregates. This cell line is widely utilized in research investigating mechanisms of drug resistance and the processes of neuroblastoma cell differentiation, as well as in the identification of novel therapeutic targets for pediatric solid tumors. |
| Tissue | Bone marrow |
| Disease | Neuroblastoma |
| Morphology | Neuroblast |
| Gender | Male |
| Age | 2 years |
| Product Format | Frozen |
| Growth Mode | Mixed: adherent and suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. High-risk neuroblastoma pathogenesis and metastasis research 2. Study of MYCN-driven oncogenic signaling and TP53-mediated drug resistance 3. Screening for novel chemotherapeutic agents and molecularly targeted therapies 4. Investigation of neuronal differentiation and apoptosis pathways 5. Development of orthotopic and subcutaneous xenograft models in nude mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in immunocompromised (nude) mice |
| Karyotype | Aneuploid; modal number = 44; multiple chromosomal rearrangements including 1p deletion |
| Genetic Profile | MYCN amplification (highly elevated); TP53 mutation (C135F); 1p36 deletion |
| Neuroendocrine Markers | Dopamine β-hydroxylase and acetylcholinesterase activities were positive. |
| Growth Kinetics | Rapid doubling time (approx. 30-40 hours); forms aggregates at high density |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove the culture medium containing suspended cells, and recover the cells via centrifugation. 2. Rinse the adherent cells with a freshly prepared 0.25% Trypsin–0.53 mM EDTA solution. 3. Add an additional 1 to 2 mL of the trypsin solution, and allow the culture flask to stand at room temperature (or 37°C) until the cells detach. 4. Add fresh culture medium, pipette the cell suspension, combine it with the previously recovered suspended cells, and aliquot the mixture into new culture flasks. |
| Medium Renewal | Every 4 to 7 days. |
| Subcultivation Ratio | A subcultivation ratio of 1:12 to 1:20 is recommended. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
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