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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SU-DHL-2 is a histiocyte cell line that was isolated in 1974 from the lymph nodes of a 73-year-old Caucasian female with large cell lymphoma. The female had diffuse histiocytic lymphoma (DHL) and after relapse was treated with chemotherapy with prednisone and chlorambucil. This cell line can be used for immunological studies. The majority of these cells are hyperdiploid with a sharp modal number of 51 chromosomes. Occasionally, cells in the hypertetraploid range can also be seen. SU-DHL-2 cells are positive for nonspecific esterase and acid phosphatase. The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-). SU-DHL-2 cells are surface Ig negative (sIG-). In addition, these cells are reported to be E-rosette negative. |
| Tissue | Lymph node |
| Disease | Large Cell Lymphoma |
| Morphology | Lymphoblast-like |
| Gender | Female |
| Age | 73 years |
| Product Format | Frozen |
| Growth Mode | Suspension, multicellular aggregates |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Research 2. Drug Screening and Development 3. Genetic Studies 4. Signal Transduction Pathway Analysis 5. Biomarker Identification 6. Immunotherapy Research 7. In Vitro Modeling |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Most of these cells were hyperdiploid, with a mode number of chromosomes up to 51, and occasionally cells in the hypertetraploid range were seen. Tiny marker chromosomes were consistently observed, and there was an increase in chromosome number in groups A, B, C, and F. |
| Immunology | CD3 -, CD10 -, CD13 -, CD20 +, CD34 -, CD37 +, CD38 -, CD80 +, CD138 -, HLA-DR - |
| Metastatic | Peritoneal effusion |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The culture can be maintained by adding fresh medium. The recommended inoculum size is 1 x 10^5 to 3 x 10^5 cells/mL. Subculture when the cell concentration is between 7 x 10^5 and 1 x 10^6 cells/mL. |
| Medium Renewal | Every 2 to 3 days. |
| Subcultivation Ratio | The ratio of 1:3 to 1:8 is recommended. |
| Culture Medium | 90% RPMI 1640 + 10% FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0596 | GFP Reporter Cell Line - SU-DHL-2 | Inquiry |
| CSC-RR0597 | Luciferase Reporter Cell Line - SU-DHL-2 | Inquiry |
| CSC-RR0598 | GFP/Luc Reporter Cell Line - SU-DHL-2 | Inquiry |
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