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MicroRNA (miRNA) deregulation is implicated in various human diseases. For studying specific miRNA functions, the widely used miRNA mimic (miR-Mimic) strategy offers transient expression. However, lentiviral vector (LV)-based miRNA expression provides stable, long-term expression, especially in challenging cell types. Creative Biogene's human and mouse precursor miRNA lentiviruses, incorporating native context sequences, offer a superior alternative to synthetic miRNAs. Co-transcribed with GFP under an optional inducible CMV promoter, these lentiviruses enable both transient and stable miRNA expression, accommodating diverse cell types for comprehensive miRNA studies.
Creative Biogene provides lentiviral vectors expressing human/mouse miRNA precursors/hairpins for sustained miRNA expression. Please browse our miRNA lentiviruses collection to find your desired one. If you don't find the miRNA lentivirus of your interest in our collection, we can also provide custom miRNA lentivirus services for your project at the best price!
Advantages of Our microRNA Lentiviral Particles
microRNA Lentiviral Particles Product List
The miR precursor lentiviruses enable stable expression in diverse cells for functional interrogation, unlike synthetic miRNAs. MiRNA precursors mimic endogenous processing into mature miRNAs while genomic context sequences ensure physiological patterns. Lentiviral integration provides persistent expression over transfection. The principle involves lentiviral transduction to integrate miR expression cassettes for potent, lasting silencing of target genes. Creative Biogene's precursor miRNA lentivirus collection offers a superior platform for establishing robust cellular miRNA models:
Case Study 1
Prostate cancer is a prevalent diagnosis in men, with African Americans (AA) facing a higher risk of developing it compared to European Americans (EA). Researchers utilized lentiviral knockdown assays to investigate miR-182's role in racial disparity in prostate cancer between AA and EA. High miR-182 expression correlated with shorter biochemical recurrence-free survival in AA patients. Knockdown of miR-182 in AA cell lines demonstrated increased apoptosis, cell cycle arrest, and inhibited colony formation, suggesting miR-182 is a potential therapeutic target for prostate cancer in AA patients. The study identified PDCD4 as a direct miR-182 target associated with aggressiveness and high Gleason grade in AA prostate cancer.
Figure 1. Researchers elucidated the role of miR-182 and its oncogenic phenotype in EA and AA cell lines. (Shiina M, et al., 2021)
Case Study 2
Researchers investigated metastasis-associated miRNA, miR-211-5p, in triple-negative breast cancer (TNBC). MiR-211-5p was downregulated in TNBC, correlating with overall survival. Its expression suppressed TNBC proliferation, invasion, and metastasis in vitro and in vivo. SETBP1 was identified as a miR-211-5p target, influencing colony and cell number. Enforced miR-211-5p expression inhibited SETBP1 and hindered TNBC progression, suggesting miR-211-5p as a potential prognostic biomarker and therapeutic target for TNBC.
Figure 2. In a xenograft model, researchers employed miR-211-5p mimic lentivirus to assess its impact on tumorigenesis and metastasis in nude mice injected with MDA-MB-231 cells. (Chen L L, et al., 2017)
What is the primary purpose of microRNA Lentiviral Particles in molecular biology research?
A: microRNA Lentiviral Particles are designed to introduce specific microRNA sequences into target cells, allowing researchers to study the regulatory functions of microRNAs in a controlled experimental setting.
Q: What role do microRNAs play in gene expression regulation, and why are they important for research?
A: Role and Importance: microRNAs are small RNA molecules that play crucial roles in post-transcriptional gene regulation. Studying microRNAs provides insights into their impact on gene expression, cellular functions, and their involvement in various diseases.
Q: In what experimental contexts are microRNA Lentiviral Particles particularly useful?
A: These particles are valuable tools for investigating the roles of specific microRNAs in cellular processes, signaling pathways, and disease models. They are widely used in functional genomics and molecular biology research.
Q: What advantages do microRNA Lentiviral Particles offer over other methods of microRNA delivery?
A: Lentiviral vectors provide efficient and stable microRNA delivery, ensuring long-term expression and allowing researchers to study the sustained impact of microRNAs on cellular processes.
| Cat.No. | Product Name | Price |
|---|---|---|
| LV00957L | Mouse Inducible tet-on miR-182 lentivirus | Inquiry |
| LV00958L | Mouse Inducible tet-on miR-183 lentivirus | Inquiry |
| LV00959L | Mouse Inducible tet-on miR-200b,a, 429 | Inquiry |
| LV00960L | Mouse Inducible tet-on miR-204 lentivirus | Inquiry |
| LV00961L | Mouse Inducible tet-on miR-211 lentivirus | Inquiry |
| LV00962L | Mouse Inducible tet-on miR-302 lentivirus | Inquiry |
| LV00976L | Mouse EF1A-miR-211 lentivirus | Inquiry |
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