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U-2 OS Cell Line
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The U-2 OS cell line, originally known as the 2T line, was cultivated from the bone tissue of a fifteen-year-old human female suffering from osteosarcoma. Established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U-2 OS cells have a very high proliferation rate and are extremely robust in experimental procedures. These make them attractive for not only cancer research but other areas of research which need cells for in vitro experiments. |
| Tissue | Bone |
| Morphology | Epithelial |
| Disease | Osteosarcoma |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Ethnicity | Caucasian |
| Gender | Female |
| Age | 15 years adult |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | U-2 OS cells have been used to assess cell proliferation using Alamar Blue and live cell imaging. |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | U-2 OS cell line is chromosomally highly altered, with chromosome counts in the hypertriploid range. |
| Images |  |
| Antigen Expression | Blood Type A; Rh+; Aw30, B12, Bw35, B40(+/-), HLA A2, |
| Receptor Expression | Insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF II) |
| Cellular Products | Osteosarcoma derived growth factor (ODGF), Blood Type A; Rh+; Aw30, B12, Bw35, B40(+/-),HLA A2 |
| Comments | U-2 OS cells exhibit epithelial adherent morphology and viruses were not detected in the line during co-cultivation with WI-38 cells or in CF tests against RSV, SV40 or adenoviruses. Mycoplasma contamination of the U-2 OS line was detected and subsequently eliminated in 1972. |
| Culture Conditions and Handling | |
| Culture Medium | DMEM + 10% FBS + 2mM L-Glutamine + 100 units/ml penicillin + 100 micro-g/ml streptomycin. |
| Thawing | 1. Take out the U-2 OS stock vial from liquid nitrogen and thaw it at room temperature. 2. Resuspend thawed cells in 10 ml growth media and transfer cells into a 10 sq. cm. tissue culture dish. Cells are grown in a 37°C incubator at 5% CO2. |
| Subculturing | 1. Aspirate growth media from the tissue culture dish. 2. Add 5 mL of Trypsin (0.05%) with EDTA solution and incubate at the 37°C incubator until cells detach. 3. Add 5 mL of fresh growth media and collect cells in a centrifuge tube. 4. Spin at 1500 rpm for 5 minutes. 5. Aspirate supernatant and add fresh growth media. 6. Transfer 1-5X10^5 viable cells/ml to a new culture vessel and incubate cultures at 37°C. |
| Split Ratio | A subcultivation ratio of 1:3 to 1:6 is recommended. |
| Medium Renewal | Every 2 to 3 days |
| Incubation Condition | Carbon dioxide (CO2), 5%; 37°C |
| Freezing | Cells can be stored as a stock in liquid nitrogen at 2-5x10^6 cells/ml in growth medium containing 5% DMSO. |
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