Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SHP-77 epithelial cells were derived from a non-encapsulated primary lung tumor in the apex of the left upper lobe of a 54-year-old Caucasian male in 1977. This cell line has unique characteristics, combining morphological features of the undifferentiated large cell variant of small cell lung cancer (SCLC) with typical biochemical properties of classical SCLC. Notably, SHP-77 cells express neural cell adhesion molecule (NCAM), NHK-1, CD56, and Leu-7 (HNK-1; CD57) on their cell surface, in addition to displaying neuroendocrine markers such as L-DOPA decarboxylase and dense core secretory granules. Electron microscopic ultrastructural analysis revealed the presence of glandular formations and intracytoplasmic lamellar bodies. They are suitable for in vitro nude mouse assays to evaluate the immunoreactivity of cells and sera from lung cancer patients. SHP-77 cells can be used as in vitro targets for 51Cr and 111 release cytotoxicity assays and in vivo nude mouse assays to evaluate the immunoreactivity of cells and sera from lung cancer patients. These cells can be used to assess the immune status of SCLC patients receiving radiation or chemotherapy. |
| Tissue | Lung |
| Disease | Carcinoma |
| Morphology | Rounded |
| Gender | Male |
| Age | 54 years |
| Product Format | Frozen |
| Growth Mode | Mixed: suspension with some loosely adherent cells |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Biology 2. Drug Screening and Development 3. Genetic Studies 4. Signal Transduction Research 5. Radiation Studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | SHP-77 cells are tumorigenic in nude mice, grow as well-defined nodules, and do not form metastases. Their doubling time is reported to be 96 hours. |
| Characteristics | |
| Genes Expressed | Neural cell adhesion molecule (NCAM) NKH-1 |
| Antigen Expression | Blood Type O; Rh +; CD56; CD57 (HNK-1,Leu-7) |
| Tumorigenic | Yes, the cells form tumors in athymic nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The culture can be maintained by adding or replacing fresh medium. Alternatively, the culture can be established by centrifugation and subsequently resuspended at a concentration of approximately 4-5 x 10^5 viable cells/mL. The cells will grow as floating clusters of cells. Some cells may adhere, but only floating cells will be transferred. |
| Medium Renewal | Every 3 to 4 days |
| Culture Medium | RPMI 1640 + 2mM Glutamine + 10% Heat Inactivated Foetal Bovine Serum (HI-FBS) |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO1124 | Human CD274/Luc Stable Cell Line - SHP-77 | Inquiry |
| CSC-RR0552 | Luciferase Reporter Cell Line - SHP-77 | Inquiry |
Copyright © Creative Biogene. All rights reserved.