sgRNA Library Screening Service

The application of CRISPR libraries makes large-scale genome editing and screening in basic research a reality. Creative Biogene helps clients unlock the full potential of their genetic research with our advanced CRISPR screening services. We offer a large number of CRISPR libraries to meet the research demands of different customers, including human whole-genome knockout libraries, mouse whole-genome knockout libraries, pathway-associated gRNA libraries, CRISPRa libraries, CRISPRi libraries, and customized gRNA libraries. Our cutting-edge technology enables high-throughput and scalable screening of genome-wide libraries for rapid identification of novel gene functions, discovery of gene interactions and exploration of complex signaling pathways.

CRISPR Library Screening Service

Introduction of CRISPR/Cas9 library Types

There are two main types of libraries used for high-throughput screening: array libraries and pooled libraries. These two types of libraries have different detection principles and characteristics. Creative Biogene's CRISPR libraries mostly use pooled libraries for high-throughput screening. Because pooled libraries are low-cost, easy to use, and can be used for in vivo studies, they provide more comprehensive genome-wide coverage, and the results can be measured after long-term culture of the infiltrated cells.

Library Types	Features
Pooled library	Pooled libraries involve computer design of sgRNA libraries to be screened and enrichment of positive clones, which are subsequently transferred to host cells to introduce various gene mutations, and the results are finally obtained by methods such as high-throughput sequencing.
Array library	Array libraries process single or several sgRNA arrays in microarrays or multi-well plates with known gene editing sequences in each well.

CRISPR/Cas9 Library Screening Procedures at Creative Biogene

Design and synthesis of sgRNA libraries
For high-throughput screening, library size is one of the key factors affecting the screening results. The genome-wide libraries we provide typically contain more than 100,000 sgRNAs to identify more genes of interest.
Construction of lentiviral vectors and infection of host cells
The sgRNA libraries are lentivirally packaged and transduced into Cas9-expressing cell lines at a low viral infection multiplicity to ensure that only 1 virus enters each cell, thus realizing the functional screening against the corresponding genes of different sgRNAs.
Positive or negative screening
According to the purpose of the study, a positive or negative screening strategy is selected.
High-throughput sequencing and bioinformatics analysis
Bioinformatics methods are utilized to find candidate genes and exclude false-positive or false-negative screening results.
Validation of candidate genes
After screening several candidate genes with CRISPR/Cas9 technology, we validated them by a series of methods to identify functional genes that regulate specific phenotypes. Our validation services include off-target analysis, individual gene knockout validation, and genetic complementation experiments.

Services Features of Our CRISPR Library Screening Service

Comprehensive and extensive libraries.
Customizable experimental design.
Robust data analysis and interpretation.

Creative Biogene's experienced specialists will work closely with our clients to develop screening programs that fit their research goals. Whether our clients need a knockout, activation or inhibition screen, we can design and deliver CRISPR libraries optimized for their specific demands. Contact us to learn more about our services.

* For research use only. Not intended for any clinical use.

Quick Inquiry