Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | XWLC-05 (Xuanwei Lung Cancer-05) is a human lung adenocarcinoma cell line derived from a 53-year-old female patient in Xuanwei County, Yunnan Province, China. The Xuanwei region is renowned for its unusually high incidence of lung cancer among non-smoking women—a phenomenon attributed to indoor air pollution resulting from the combustion of "smoky coal." XWLC-05 exhibits an epithelial cell morphology and serves as a specialized model for investigating the unique genomic characteristics and environmental etiology of "Xuanwei-type" lung cancer. This cell line is characterized by the presence of specific somatic mutations and epigenetic alterations associated with polycyclic aromatic hydrocarbons (PAHs). As an indispensable research tool, this cell line plays a pivotal role in fields such as the mechanistic study of non-smoking-related carcinogenesis, the genetics of cancer within specific populations, and the screening of targeted therapeutic agents for distinct subtypes of lung adenocarcinoma. |
| Tissue | Lung |
| Disease | Lung Adenocarcinoma (Xuanwei-type) |
| Morphology | Epithelial-like |
| Gender | Female |
| Age | 53 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on environmental carcinogenesis and PAH-induced lung cancer 2. Study of the unique genetic profile of lung cancer in the Xuanwei population 3. Drug screening and sensitivity testing for lung adenocarcinoma 4. Investigation of tumor cell proliferation, migration, and signaling 5. Development of human tumor xenograft models for regional cancer studies |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Karyotype | Aneuploid; complex chromosomal rearrangements |
| Genetic Profile | Characteristic of environmental-related lung cancer; often used to study EGFR, KRAS, and TP53 status in specific regional contexts |
| Source Context | Derived from a patient with high exposure to domestic coal smoke |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically requiring 3 to 8 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00859 | GFP Reporter Cell Line - XWLC-05 | Inquiry |
Copyright © Creative Biogene. All rights reserved.