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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | 22Rv1 is a xenograft-derived human prostate cancer epithelial cell line that was serially propagated in mice following castration-induced regression and recurrence of parental androgen-dependent CWR22 xenografts. The 22Rv1 cell line expresses androgen receptor (AR) and prostate-specific antigen (PSA), both markers of prostate cancer. The presence of these markers in 22Rv1 cells confirms their origin from prostate cancer tissue and emphasizes their relevance in studying this disease. Importantly, the 22Rv1 cell line is unique in that it expresses both full-length and truncated forms of AR. This mixed expression pattern is commonly seen in androgen deprivation-resistant prostate cancer, making the 22Rv1 cell line a valuable model for studying mechanisms of resistance to hormone therapy. |
| Tissue | Prostate |
| Disease | Carcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (2 when used for genetic engineering) Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Prostate cancer research 2. Drug testing 3. Genetics research 4. Protein expression studies 5. Tumor growth studies 6. 3D cell culture |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The 22Rv1 cell line exhibits sensitivity to enzalutamide, the standard of care therapy for patients with advanced prostate cancer. Enzalutamide is an androgen receptor antagonist that inhibits tumor growth by inhibiting the binding of androgens to receptors. The response of 22Rv1 cells to enzalutamide highlights the clinical relevance of this cell line and its potential to evaluate therapeutic interventions targeting androgen receptor signaling. |
| Characteristics | |
| Antigen Expression | Prostate-specific antigen (PSA) |
| Expression Markers | Androgen receptor |
| Tumorigenic | Yes, forms tumors in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old culture medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, resuspend cells by gently mixing with 10 ml of medium and centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:3 to 1:6 is recommended. |
| Culture Medium | RPMI 1640 without phenol red + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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