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Mutagenesis in the laboratory is an important molecular biology technique that is used to make specific and intentional changes to the DNA sequence of a gene and any gene products. It is used for studying protein structure-function relationships, altering protein activity, and for modifying vector sequences to incorporate affinity tags and correct frame shift errors. So far, large number of methods for achieving mutagenesis has been developed and can be divided into two main types, random or site-directed.
Site-directed mutagenesis is one of the most important techniques in laboratory for introducing a mutation into a DNA sequence. With the help of this strategy researchers are able to rapidly and efficiently create point mutations, amino acid substitutions, insertions, and deletions in virtually any double-stranded plasmid-useful for understanding protein-protein interactions and structure-function relationships.
Random mutagenesis is a powerful tool for elucidating protein structure-function relationships and for modifying proteins to improve or alter their characteristics. With random mutagenesis, point mutations are introduced at random positions in a gene-of-interest, typically through an error-prone DNA polymerase reaction (error-prone PCR). The mutated PCR products are then cloned into an expression vector and the resulting mutant library can be screened for changes in protein activity. Random mutagenesis allows researchers to identify beneficial mutations in the absence of structural information, or when such mutations are difficult to predict from protein structure.
As one of the leading biotechnology company in the world, Creative Biogene is able to provide a series of Mutagenesis Kits, including site-directed mutagenesis kit and random mutagenesis kits. Creative Biogene’s goal is to provide you high-quality product to accelerate your researches in investigating the structure and biological activity of DNA, RNA, and protein molecules, and/or protein engineering.