Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
microRNA inhibitors are complementary, antisense single-stranded oligonucleotides to their target, endogenous mature miRNA. Synthetic miRNA inhibitor designs incorporate the reverse complement of the mature miRNA (the target site) and are chemically modified to prevent RISC-induced cleavage, enhance binding affinity and provide resistance to nucleolytic degradation. When delivered to a cell, binding of endogenous mature miRNAs to these complementary synthetic target sites is thought to be irreversible, therefore these inhibitors are presumed to sequester the endogenous miRNA, making it unavailable for normal function.
Creative Biogene can speed up your research by providing high-quality miRNA inhibitors. Our miRNA inhibitors are the single-stranded synthetic inhibitor targeting all human miRNAs in the miRBase Sequence Database. These miRNA inhibitors are available at 5, 10 and 20 nmol guaranteed yield.
Please browse our miRNA inhibitors collection to find your desired one. If you don't find the miRNA inhibitors of your interest in our collection, we can also work for your project with the best price!
miRNA inhibitors work by silencing or inhibiting the activity of specific miRNAs, effectively inhibiting their regulatory effects on gene expression. miRNA inhibitors are important tools for perturbing microRNA function and assessing the phenotypic consequences in gain or loss of function assays. miRNA inhibitors can be used in conjunction with miRNA and gene expression profiling to identify specific miRNA-gene target relationships. In addition, several screens have been performed that implicated miRNAs in biological systems such as cancer and viral infections. By targeting these dysregulated miRNAs with inhibitors, normal gene expression patterns can be restored and tumor growth halted. Therefore, miRNA inhibitors particularly show promise in therapeutic development.
Applications for miRNA inhibitors include:
Case Study 1
Here, researchers evaluated the impact of miR-1225 expression on pancreatic cancer (PC) generation and progression. The expression of miR-1225 is upregulated in PC cells and PC patient specimens. Furthermore, inhibition of miR-1225 impaired PC cell proliferation in vitro and in vivo and inhibited tumor growth. Reduction in miR-1225 expression leads to induction of apoptosis in PC cells. These results indicate that PC cells utilize miR-1225 to inhibit apoptosis by reducing JAK1 expression.
Figure 1. Effect of miR-1225 expression on the proliferation of PANC-1 and AsPC-1 cells. (a,b) Expression of miR-1225 in PANC-1 and AsPC-1 cells transfected with miR-1225 inhibitor or NC inhibitor, measured by qPCR. (c, d) Proliferation rates of PANC-1 and AsPC-1 cells were measured at 24, 48, and 72 h post-transfection using EdU assay. (e,f) Soft agar colony formation assay of PANC-1 and AsPC-1 cells transfected with miR-1225 inhibitor or NC inhibitor. (The miR-1225 inhibitor and NC inhibitor were obtained from Creative Biogene) (Zhong R, et al. 2019)
Case Study 2
Here, researchers investigated how miR-506 is involved in retinoblastoma (RB) production. Upregulation of MiR-506 expression was found in RB cells and RB patient specimens. Inhibition of MiR-506 impairs RB cell proliferation and inhibits tumor growth in vitro and in vivo. Reduction in miR-506 expression leads to induction of apoptosis in RB cells (WERI-Rb1 and Y79). These results indicate that miR-506 inhibits apoptosis by inhibiting the expression of SIRT1 in RB cell lines.
Figure 2. Effect of miR-506 expression on RB1 and Y79 cell proliferation. (A, B) Examination of miR-506 expression in RB1 and Y79 cells transfected with miR-506 inhibitor or NC inhibitor by qPCR. (C,D) Proliferation rates of RB1 and Y79 cells obtained at 24, 48, and 72 h posttransfection using EdU assay. (E, F) Colony formation assay of RB1 and Y79 cells transfected with miR-506 inhibitor or NC inhibitor. (The miR-506 inhibitor and NC inhibitor were obtained from Creative Biogene.) (Song Z, et al. 2019)
Q: What is miRNA inhibitor?
A: miRNA inhibitors are small RNA molecules that can bind to and inhibit the activity of specific microRNAs (miRNAs).
Q: How does miRNA inhibitor work?
A: miRNA inhibitors are designed to have complementary sequences to the specific microRNA they target. When introduced into the cell, the inhibitor binds to the microRNA, forming a stable complex that prevents it from interacting with its target mRNA molecules.
Q: How do miRNA inhibitors from Creative Biogene perform compared to other chemically synthesized inhibitors?
A: Creative Biogene's miRNA inhibitors have superior potency, long-lasting inhibition, and extremely low cell toxicity compared to other chemically synthesized miRNA inhibitors.
Q: How are the expression cassettes for miRNA inhibitors designed?
A: The expression cassettes have been designed using a proprietary algorithm and tested rigorously by carefully designed experiments.
| Cat.No. | Product Name | Price |
|---|---|---|
| MINH0010 | miR-hsa-miR-1 inhibitors | Inquiry |
| MINH0011 | miR-hsa-miR-7-1-3p inhibitors | Inquiry |
| MINH0012 | miR-hsa-miR-7-2-3p inhibitors | Inquiry |
| MINH0013 | miR-hsa-miR-7-5p inhibitors | Inquiry |
| MINH0014 | miR-hsa-miR-9-3p inhibitors | Inquiry |
| MINH0015 | miR-hsa-miR-9-5p inhibitors | Inquiry |
| MINH0016 | miR-hsa-miR-10b-3p inhibitors | Inquiry |
| MINH0017 | miR-hsa-miR-10a-3p inhibitors | Inquiry |
| MINH0018 | miR-hsa-miR-10b-5p inhibitors | Inquiry |
| MINH0019 | miR-hsa-miR-10a-5p inhibitors | Inquiry |
| MINH0020 | miR-hsa-miR-15a-3p inhibitors | Inquiry |
| MINH0021 | miR-hsa-miR-15b-3p inhibitors | Inquiry |
| MINH0022 | miR-hsa-miR-15a-5p inhibitors | Inquiry |
| MINH0023 | miR-hsa-miR-15b-5p inhibitors | Inquiry |
| MINH0024 | miR-hsa-miR-16-2-3p inhibitors | Inquiry |
Copyright © Creative Biogene. All rights reserved.