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Design and synthesis of mRNA molecules is a prerequisite for downstream production of mRNA. Creative Biogene offers custom mRNA design and synthesis services to meet our customers' GMP mRNA production demands. Our high-quality synthesis services are ideal for the subsequent production of mRNA for functional studies, therapeutics, diagnostics and vaccine production.
The design and synthesis of mRNA is an important part of mRNA production. The structure of mRNA can be divided into five components, including the 5'cap, the 5' and 3' untranscriptional region (UTR), the open reading frame for the gene of interest, and the poly(A) tail. Each component is crucial to the stability and quality of the final product. The mRNA design and synthesis process begin with codon optimization of the sequence for optimal transcription. The gene was synthesized, cloned into a vector, and validated by Sanger sequencing. The plasmid DNA was then amplified and linearized against the target mRNA sequence for subsequent in vitro transcription of the mRNA.
Fig. 1 Structure of mRNA. (Chaudhary, N. et al. 2021)
Creative Biogene, with years of experience in mRNA production, offers efficient mRNA design and synthesis services for use as the optimal template for in vitro transcription. Our custom sequence-based design produces high-quality mRNA. our strong manufacturing capabilities allow us to accelerate your GMP mRNA production by providing the following services for your gene of interest:
The mRNA sequence design mainly includes codon optimization, 5'UTR selection and 3'UTR selection. Creative Biogene provides fast and efficient mRNA design portfolio strategies to facilitate GMP mRNA production. Our advanced tools for rapid design and screening of mRNA can systematically determine the impact of UTR influence, codon sequence and RNA structure on mRNA stability and translation efficiency, thus more rapidly and efficiently helping customers to obtain the ideal mRNA sequence for optimal transcription.
Creative Biogene's team with years of experience in gene synthesis screens, optimizes and synthesizes the target sequence with 99% reliability of project delivery.
Creative Biogene's experienced scientists perform cloning and plasmid preparation services for you, helping you create custom plasmid constructs for downstream applications as needed for your project, and validate them with accuracy and reliability.
Creative Biogene utilizes our advanced production platform to purify the resulting plasmids, detect contaminants, and treat them with restriction endonucleases to linearize the plasmid DNA templates for downstream mRNA production.
Creative Biogene offers custom mRNA design and synthesis services. Our flexible project execution capabilities allow for custom mRNA synthesis services for sequences from the following sources:
| Freeze-dried plasmid DNA | CoA documents | Sequencing mapping |
| Sequence comparison chart | Plasmid structure diagram |
Creative Biogene has developed an advanced mRNA design and synthesis service platform for the synthesis of arbitrarily simple or difficult gene sequences. Our advanced development capabilities can provide optimal transcriptional templates for subsequent GMP mRNA production to accelerate the development of our customers' mRNA products or therapeutics. Please feel free to contact us if you have a need, and our experienced specialists will be happy to assist you.
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