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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SU-DHL-1 is a histiocytic cell that was isolated from the lymph node of a White, 10-year-old, male patient with large cell lymphoma. These cells consistently failed to express T or B cell markers, even after they were induced by phorbol ester (TPA), retinoic acid, dimethylsulfoxide (DMSO), or heme. The SU-DHL-1 cells lack immunoglobulin gene rearrangements. They demonstrate cytoplasmic acid phosphatase and nonspecific esterase (ANAE) activities, and they can be stained with a monoclonal antibody (MoAb), anti-Leu M5, that normally reacts with monocytes and histiocytes. |
| Tissue | Lymph node |
| Disease | Large Cell Lymphoma |
| Morphology | Lymphoblast-like |
| Gender | Male |
| Age | 10 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer Research 2. Drug Testing and Development 3. Gene Studies 4. Immunology Research 5. Biomarker Identification 6. In Vitro Modeling |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | SU-DHL-1 cells are surface Ig negative (sIG-). SU-DHL-1 cells are non-specific esterase, acid phosphatase and oil red O positive. SU-DHL-1 cells are periodic acid Schiff negative. These cells phagocytose Candida albicans and latex particles. |
| Characteristics | |
| Karyotype | 88 chromosomes; t(2;5)(p23;q35) translocation |
| Immunology | CD3 -, CD5 +, CD10 -, CD13 -, CD19 -, CD20 -, CD25 +, CD30 +, CD34 -, CD37 -, CD38 -, CD71 +, CD80 -, CD138 -, HLA-DR -, sm/cyIgM -, sm/cyIgG -, sm/cykappa -, sm/cylambda - |
| Genes Expressed | Fusion gene NPM-ALK (p80)+; Anaplastic lymphoma kinase (ALK)+; Lg not expressed |
| Expression Markers | CFS-1, expressed; (IL-1-R; IL-2-Rα; IL-6-R; TNFα-R; c-fms), expressed; Surface Receptors: (Fc; IgMEAC; IgGEA; & E), not expressed |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The culture can be maintained by adding fresh medium. The recommended inoculum size is 8.0 x 10^4 to 2.0 x 10^5 cells/mL. Subculture when the cell concentration is between 1.0 x 10^6 and 1.5 x 10^6 cells/mL. |
| Medium Renewal | Every 2 to 3 days. |
| Subcultivation Ratio | The ratio of 1:4 to 1:12 is recommended. |
| Culture Medium | 90% RPMI 1640 + 10% FBS |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃ |
| Cryopreservation | Complete culture medium supplemented with 20% FBS and 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0593 | GFP Reporter Cell Line - SU-DHL-1 | Inquiry |
| CSC-RR0594 | Luciferase Reporter Cell Line - SU-DHL-1 | Inquiry |
| CSC-RR0595 | GFP/Luc Reporter Cell Line - SU-DHL-1 | Inquiry |
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