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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | SK-BR-3 (also known as SkBr3) is a human breast cancer cell line isolated in 1970 at Memorial Sloan-Kettering Cancer Center for use in therapeutic research, specifically HER2-targeted therapy. SK-BR-3 cells were derived from pleural effusion arising from adenocarcinoma in a 43-year-old white woman. The cell line harbors genetic aberrations commonly seen in breast cancer, including HER2 gene amplification and mutations in the p53 tumor suppressor gene. Overexpression of HER2 in SK-BR-3 cells makes it a valuable model for studying HER2-positive breast cancer, which is characterized by aggressive growth and poor prognosis, as well as HER2-targeted therapies. SK-BR-3 cells played an important role in the study of trastuzumab (Herceptin), a monoclonal antibody directed against HER2 that has become a cornerstone of treatment for HER2-positive breast cancer. |
| Tissue | Breast, mammary gland |
| Disease | Invasive ductal carcinoma |
| Morphology | Epithelial-like |
| Gender | Female |
| Age | 43 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Drug testing 2. Genetic studies 3. Protein research 4. Herceptin research 5. Pathway analysis |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The SK-BR-3 cell line is autologous (derived from the same patient) to the AU565 cell line. These cells are considered biosafety level 1. They are known to grow in grape-like clusters and have an invasive phenotype similar to cells in vivo. SK-BR-3 cells have been used in studies seeking to overcome Herceptin therapeutic resistance in HER2-overexpressing breast cancer. The cell line has also been tested for applications such as CRISPR/Cas9 gene editing, antibody resistance in transfection, and HER2-based cancer therapy in the context of microenvironmental fluctuations. |
| Characteristics | |
| Karyotype | This is a hypertriploid human cell line with a modal chromosome number of 84, present in 34% of the cells. Cells with 80 chromosomes were also present in a high proportion (28%); the proportion of higher ploidy cells was 7.3%. This cell line has a very complex chromosomal composition. Between 35% and 40% of the chromosomes in the complement of cells with a chromosome mode of 84 consist of structurally altered marker chromosomes. Several markers are longer than chromosome N1. |
| Protein Expression | p53 positive |
| Antigen Expression | Blood Type A, Rh+, HLA A11, Bw22(+/-), B40, B18 |
| Tumorigenic | Yes, in nude mice, forms poorly differentiated adenocarcinoma |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old culture medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, resuspend cells by gently mixing with 10 ml of medium and centrifuge at 300xg for 3 minutes. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | DMEM supplemented with 4.5g/L glucose, 1.5 mM L-glutamine and 10% fetal bovine serum |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37°C |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0158 | Cas9 Stable Cell Line - SK-BR-3 | Inquiry |
| CSC-RR00712 | Luciferase Reporter Cell Line - SK-BR-3 | Inquiry |
| CSC-RR00873 | GFP/Luc Reporter Cell Line - SK-BR-3 | Inquiry |
| CSC-RR0280 | GFP Reporter Cell Line - SKBR3 | Inquiry |
| CSC-RR0287 | RFP Reporter Cell Line - SK-BR-3 | Inquiry |
| CSC-RT2673 | Human SENP7 Knockout Cell Line-SK-BR-3 | Inquiry |
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