Daudi Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	The Daudi cell line was established in 1967 from a 16-year-old African boy diagnosed with Burkitt's lymphoma, a type of lymphoma. Named after the patient from whom it was derived, the Daudi cell line is characterized by Epstein-Barr virus (EBV) positivity, a common feature of Burkitt's lymphoma and several other lymphoproliferative disorders. EBV infection in these cells provides a unique model for studying the role of the virus in tumorigenesis, particularly in B-cell malignancies. A notable feature of the DAUDI cell line is the presence of c-Myc translocations, specifically the t(8;14)(q24;q32) translocation. This translocation results in constitutive upregulation of the MYC gene, which results in overexpression of β-catenin. Dysregulation of the β-catenin signaling pathway has been implicated in the induction of stemness in cancer cells. Therefore, the DAUDI cell line provides an opportunity to study the molecular mechanisms underlying the development and progression of Burkitt's lymphoma, particularly the role of c-Myc and β-catenin in promoting stemness and tumorigenic potential.
Disease	Burkitts lymphoma
Morphology	Lymphoblast
Gender	Male
Age	16 years
Product Format	Frozen
Growth Mode	Suspension
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Cancer Research 2. Immunology Studies 3. Vaccine Development 4. Drug Screening 5. Virology Research
Shipped In	Dry ice
Storage Temperature	−196°C
Additional Info	Daudi human cells lack expression of classical major histocompatibility complex (MHC) class I molecules on their surface due to the absence of β-2-microglobulin, a key component responsible for the correct intracellular folding and processing of MHC class I molecules in the endoplasmic reticulum. The lack of β-2-microglobulin in the Daudi cell line results in a lack of glycosyl modifications necessary for the proper expression of these molecules on the cell surface.
Characteristics
Karyotype	46, almost diploid
Tumorigenic	Yes, in nude mice
Antigen Expression	CD10+, CD19+, CD20+, CD21+, CD22+, CD23-, CD24-, CD32+, CD37+, CD38+, CD39-,CD40+, CD54+, CD72+, CD73-, CD75+, CD77+, CD81+, CD82+, CD83-, CD84+, CD86+
Expression Markers	Complement, expressed; Fc, expressed
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	The culture can be maintained by adding fresh medium or replacing the medium. Alternatively, the culture can be established by centrifugation and subsequently resuspended at a density of 3 to 5 x 10⁵ viable cells/mL. Maintain cell density between 3 x 10⁵ and 2 to 3 x 10⁶ viable cells/mL. T-75 flasks are recommended for subculturing this product.
Medium Renewal	Add fresh medium every 2 to 3 days.
Culture Medium	RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: Air, 95%; CO₂, 5%; Temperature: 37℃
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO0209	Human PDCD1 Stable Cell Line - Daudi	Inquiry
CSC-RR0566	GFP Reporter Cell Line - Daudi	Inquiry
CSC-RR0567	Luc Reporter Cell Line - Daudi	Inquiry
CSC-RR0568	GFP/Luc Reporter Cell Line - Daudi	Inquiry

* For research use only. Not intended for any clinical use.

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