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Understanding neuronal connectivity, signal transmission, and the functional position of specific neuron populations is critical in neuroscience research. Traditional tracers, such as fluorescent dyes or molecular probes, are often limited in multi-synaptic or cross-layer network mapping due to poor diffusion, weak signals, or low specificity. Viral tracing tools, with their self-replicating and transneuronal properties, have become essential for comprehensive neural circuit studies.
Creative Biogene offers high-quality RABV (Rabies Virus) vector services to support advanced neural tracing applications. Our services encompass design, packaging, purification, titer determination, and technical support, ensuring researchers can focus on scientific insights rather than technical hurdles.
Among neurotropic viruses, RABV is widely used due to its natural retrograde, trans-synaptic transmission, relatively low toxicity, and minimal disruption to host neuron metabolism. Certain modified RABV strains allow controlled retrograde propagation between connected neurons, making RABV a uniquely directional transneuronal tracer.
Figure 1. The RABV transcription and replication strategy. (Davis BM, et al., 2015)
RABV is a negative-sense single-stranded RNA virus (~12 kb), encoding five structural proteins: N (nucleoprotein), P (phosphoprotein), M (matrix), L (RNA-dependent RNA polymerase), and G (glycoprotein). The G glycoprotein mediates neuron-specific entry and retrograde transport. For tracing applications, the glycoprotein gene is often deleted (G-deleted) to block autonomous budding and synaptic spread. By providing G protein in trans or using pseudotyping strategies, the virus can be controlled for monosynaptic tracing, propagation efficiency, and network targeting. Second- and third-generation RABV vectors further reduce cytotoxicity and extend labeling windows.
Creative Biogene provides tailored RABV solutions that simplify viral vector design, production, and delivery, enabling researchers to focus on neuroscience discoveries.
Highly Customizable
Incorporation of fluorescent markers (GFP, RFP) or functional genes.
Controlled Trans-Synaptic Spread
G-deleted virus, G complementation, or pseudotyping allows precise monosynaptic tracing.
Low Cytotoxicity Options
Optimized strains maintain efficient tracing while minimizing neuron damage.
Stringent Quality Control
Viral concentration, purification, titer measurement, and functional verification ensure reliable and safe reagents.
1Vector Construction: The G-deleted RABV genome is constructed in plasmids, with insertion of target transgenes and optional regulatory elements.
2Virus Rescue: Transfection of the RABV genome and helper proteins (N, P, L, G) into packaging cells generates the initial virus. Pseudotyping (e.g., EnvA) is performed if needed.
3Amplification and Packaging: The Initial virus is expanded in G-expressing cells, followed by pseudotyping as required.
4Purification: High-purity viral particles are obtained via centrifugation, chromatography, or filtration, removing cell debris and contaminants.
5Titer Measurement and QC: Infection titer (TCID₅₀, pfu/mL, ffu/mL) and properties such as infection efficiency and trans-synaptic specificity are verified.
6Delivery & Technical Support: Virus is shipped under appropriate cold-chain conditions with safety instructions, usage guidelines, and optional experimental consultation.
Although G-deleted vectors are replication-deficient, strict biosafety protocols must be followed. Users should monitor infection windows to avoid neuronal damage, minimize non-specific infection, and adhere to institutional biosafety level requirements. Creative Biogene provides detailed handling instructions and recommends approval by the relevant biosafety committee.
Creative Biogene's RABV tracing services provide a complete solution from vector design to delivery and technical support. Our platform enables single- or multi-synaptic tracing and integration of functional elements, helping researchers focus on scientific discoveries rather than technical bottlenecks.
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