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Rabies Virus (RABV) Service

Creative Biogene is offering rabies virus (RABV) services to promote RABV-based neuronal tracing studies.

RABV Introduction

Rabies Virus (RABV) is a type of enveloped, bullet-shaped, negative-strand RNA virus in the genus of Lyssavirus of the family Rhabdoviridae. The RNA genome of the virus is approximately 12 kb in length and encodes five viral proteins which includes nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and the viral RNA polymerase (L).

Rabies Virus (RABV) Figure 1. Rabies Virus (RABV)

RABV propagates exclusively between connected neurons in a retrograde direction. After entering one cell, RABV replicates and nascent RABVs retrogradely infect neurons that project to the starter cell. Subsequently, RABVs spread through higher and higher order neural connections. However, since wild-type RABV spreads across multiple synapses, it doesn’t reveal clear input and output connections between RABV labeled neurons. To overcome this practical limitation, RABV has been modified to selectively infect starter cell types of interest as well as to monosynaptically spread. Modified RABV is becoming an important molecular tool in the area of investigating how neurons are connected.

RABV Design & Production

It has shown that the RABV glycoprotein (G) protein is necessary and sufficient for retrograde transmission. As a neural tracing molecular tool, RABV is usually engineered to be G protein deleted and (or) EnvA-pseudotyped. Scientists at Creative Biogene have mastered knowledge and molecular technologies in manipulating RABV RNA genome and are able to develop RABVs that can be used in neural tracing. We also offer premade RABVs which include RABV-ΔG-GFP, RABV-ΔG-RFP, RABV-EnvA-ΔG-GFP and RABV-EnvA-ΔG-RFP. If you are looking for RABV assistance, welcome to contact us at 1-631-626-9181 or .

Red fluorescent image of cells infected with RABV-ΔG-RFP. Figure 2. Red fluorescent image of cells infected with RABV-ΔG-RFP.

Applications

  • Targeting specific brain regions
  • Monosynaptic tracing in two specific brain regions

Our Service Includes

Service workflow Figure 3. Service workflow

Generation of G-deleted rabies virus (RABV-ΔG) consist of the following steps:
(a) construction of RABV genome
(b) recovery of RABV from plasmids
(c) RABV amplification
(d) RABV pseudotyping
(e) RABV concentration and titration

References

  1. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study. J Gen Virol. 2000 Sep;81(Pt 9):2147-53.
  2. Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors. Nat Protoc. 2013 Aug;8(8):1583-601.
  3. Callaway EM, Luo L. Monosynaptic Circuit Tracing with Glycoprotein-Deleted Rabies Viruses. J Neurosci. 2015 Jun 17;35(24):8979-85.

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