Site-saturation Mutagenesis Library Construction Service
Creative Biogene, as one of the leading biotechnology companies, can offer the dedicated service for the site-saturation mutagenesis library construction. Creative Biogene possesses sophisticated equipments, advanced technologies and highly experienced staffs which are available to provide site-saturation mutagenesis library construction service to customers worldwide. Creative Biogene's goal is to provide you with the most affordable, high-quality site-saturation mutagenesis library construction service to ensure your satisfaction in a timely and professional manner.

Site-saturation mutagenesis (SSM) is a simple, semi-rational approach to protein engineering that occupies a useful middle ground between fully rational site-directed mutagenesis strategies and non-rational mutagenesis strategies. In SSM, the semi-rational designation derives from the fact that target residues are identified in a specific, knowledge-guided manner, but are then mutated randomly, in either an individual or combinatorial fashion. Through the site-saturation mutagenesis, it is possible to create a library of mutants containing all possible combinations of 20 different amino acids at one or more predetermined positions in a gene sequence. SSM is a powerful approach in protein engineering to characterize structure-function relationships, as well as to create improved protein variants.
Applications of site-saturation mutagenesis
- Generate novel protein variants
- Directed evolution;
- Structure–function studies
- Fine-tune enzyme properties including substrate specificity, thermostability or simply to increase activity.
Creative Biogene possesses extensive expertise and experience which is able to provide you custom site-saturation mutagenesis library construction service. Creative Biogene's top priorities are to streamline the platform processes or work with you to design a process that meets your specific needs. Creative Biogene is a reliable partner that can work with you to accelerate the speed to achieve your research goals. If you have any special requirements in our site-saturation mutagenesis library construction service, please feel free to contact us. We are looking forward to working together with your attractive projects.
References:
- Siloto R M & Weselake R J. (2012). Site saturation mutagenesis: Methods and applications in protein engineering. Biocatalysis and Agricultural Biotechnology, 1(3), 181-189.
- Williams E M, et al. (2014). Site-saturation mutagenesis by overlap extension PCR. InDirected Evolution Library Creation (pp. 83-101). Springer, New York, NY.
- Kretz K A, et al. (2004). Gene site saturation mutagenesis: a comprehensive mutagenesis approach. In Methods in enzymology (Vol. 388, pp. 3-11). Academic Press.
* For research use only. Not intended for any clinical use.