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Lentiviruses, part of the retroviridae family, are widely used for stable cell line establishment due to high transduction efficiency, low immunogenicity, and durable expression. Unlike gammaretroviruses, lentiviruses infect non-replicating cells, making them powerful tools for gene function studies. Creative Biogene offers a diverse premade lentiviral collection with human, rat, and other organisms' genes, tagged or reporter options. Our ready-to-use lentiviruses efficiently deliver specific target expression to various cell types, including primary and non-dividing cells. For customized projects, utilize our top-notch lentiviral services.
Key Features of Our Gene-specific Lentiviral Particles
Gene-Specific Recombinant Lentiviruses Product List
The gene-specific lentiviral vectors enable simple, rapid gain-of-function studies by efficiently transducing even hard-to-transfect cells to induce sustained overexpression. The principle involves cloning full-length ORFs into optimized lentiviral transfer vectors for maximal stable delivery. The ready-to-use lentiviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts. We provide powerful customizable tools for diverse functional genomics applications from cell lines to in vivo:
Case Study 1
Researchers employed lentiviral vectors to investigate the impact of Glaucocalyxin A (GLA) on gastric cancer (GC). GLA-induced apoptosis in GC cells, involving increased caspase-3 activity and elevated levels of cleaved caspase-3 and cleaved PARP. The downregulation of Murine Double Minute Clone 2 (MDM2) and Ring Finger Protein 6 (RNF6) by GLA was attributed to miR-3658, which GLA upregulated through demethylation and abrogation of EZH2-mediated epigenetic silencing. The study indicates GLA's therapeutic potential for GC through posttranscriptional regulation of oncogenes.
Figure 1. GLA treatment significantly downregulated oncogenes Mdm2 and RNF6 in gastric cancer cells, as identified in our previous gene regulation screening. Human MDM2 and RNF6 lentivirus particles were from Creative Biogene, New York, USA. (Liu Y, et al., 2022)
Case Study 2
Ewing sarcomas commonly develop in the pelvic and stylopod bones, such as the femur and humerus, possibly due to the transformation of mesenchymal stem/progenitor cells (MSCs) induced by the EWS-FLI1 oncogene. Researchers utilized lentiviral transduction to investigate the impact of EWS-FLI1 on embryonic superficial zone (eSZ) cells expressing posterior Hox genes in Ewing sarcoma tumorigenesis. They isolated eSZ cells from various bones, revealing that stylopod eSZ cells are more susceptible to EWS-FLI1-induced transformation, consistent with the predominant presentation of Ewing sarcoma in pelvic and stylopod bones. Surprisingly, loss of Hoxd13 had no impact on EWS-FLI1-induced transformation, indicating its dispensability in mouse MSCs. These findings underscore anatomically-defined differences in MSCs contributing to the distinct susceptibility of skeletal sites to Ewing sarcoma.
Figure 2. Researchers successfully isolated Hox+ eSZs from zeugopod and stylopod, demonstrating their tolerance to lentiviral-mediated EWS-FLI1 expression. (Pfaltzgraff ER, et al., 2019)
Q: What types of genes can be introduced using Gene-Specific Lentiviral Particles?
A: These particles can be customized to introduce a wide range of genes, including those encoding proteins of interest, RNA molecules, or other genetic elements for precise manipulation of cellular functions.
Q: In what experimental contexts are Gene-Specific Lentiviral Particles particularly useful?
A: These particles are valuable tools for functional genomics, target validation, pathway analysis, and investigating the roles of specific genes in various cellular processes.
Q: What are the advantages of using lentiviral vectors for gene delivery in comparison to other methods?
A: Lentiviral vectors offer efficient and stable gene delivery, integrate into the host genome for long-term expression, and are suitable for both dividing and non-dividing cells, making them a preferred choice for many gene-specific applications.
Q: Are Gene-Specific Lentiviral Particles compatible with various cell types, including primary cells and cell lines?
A: Yes, these particles are versatile and can be used with a wide range of cell types, including primary cells and established cell lines, making them suitable for diverse research scenarios.
| Cat.No. | Product Name | Price |
|---|---|---|
| LV00014Z | Human NGFR lentiviral particles | Inquiry |
| LV00060Z | Human BCL6 lentiviral particles | Inquiry |
| LV00061Z | Human BRF1 lentiviral particles | Inquiry |
| LV00062Z | Human CAB39 lentiviral particles | Inquiry |
| LV00063Z | Human CEBPA lentiviral particles | Inquiry |
| LV00064Z | Human CEBPB lentiviral particles | Inquiry |
| LV00065Z | Human CEBPD lentiviral particles | Inquiry |
| LV00066Z | Human CREBBP lentiviral particles | Inquiry |
| LV00067Z | Human FOXO3 lentiviral particles | Inquiry |
| LV00068Z | Mus musculus Mmp3 lentiviral particles | Inquiry |
| LV00069Z | Human PRDM1 lentiviral particles | Inquiry |
| LV00071Z | Human PRKAA2 lentiviral particles | Inquiry |
| LV00073Z | Human SIRT1 lentiviral particles | Inquiry |
| LV00074Z | Mus musculus Sirt1 lentiviral particles | Inquiry |
| LV00075Z | Human SIRT2 lentiviral particles | Inquiry |
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