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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | ID8 is an ovarian surface epithelial (OSE) cell line derived from C57BL/6 mice. It was established through spontaneous malignant transformation of primary OSE cells after repeated in vitro passages. ID8 is currently the most widely used homogeneous mouse epithelial ovarian cancer (EOC) model. It effectively mimics high-grade serous ovarian cancer in humans, particularly demonstrating rapid formation of large amounts of ascites and multiple metastatic nodules after intraperitoneal injection. This cell line is a cornerstone of immunological and oncological research, used to study the ovarian tumor microenvironment and test novel immunotherapies. |
| Tissue | Ovary |
| Disease | Ovarian Epithelial Carcinoma |
| Morphology | Epithelial-like |
| Gender | Female |
| Age | Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Homologous mouse models for ovarian cancer research 2. Studies on tumor-associated immunosuppression and T-cell exhaustion 3. Studies on peritoneal metastasis and ascites formation 4. Preclinical trials of vaccines and immune checkpoint inhibitors 5. Evaluation of the vascular endothelial growth factor (VEGF) signaling pathway |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic in syngeneic C57BL/6 mice |
| Metastatic Potential | High potential for peritoneal dissemination and ascites development |
| Genetic Profile | Wild-type p53 (though often modified in research sublines like ID8-p53-/- to better mimic human HGSOC) |
| Expression Markers | Cytokeratin positive; Low expression of mesothelin |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca²⁺/Mg²⁺-free PBS buffer to remove residual serum. 3. Add 2.0 mL of 0.25% trypsin-0.53 mM EDTA solution and incubate at 37°C for 3 to 5 minutes until cells detach. 4. Neutralize with complete culture medium and gently pipette to prepare a single-cell suspension. 5. Aliquot the cells into new culture dishes. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:4 to 1:8 is recommended |
| Culture Medium | DMEM supplemented with 4% to 10% Fetal Bovine Serum (FBS), 5 μg/mL Insulin, 5 μg/mL Transferrin, and 5 ng/mL Sodium Selenite. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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