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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | The HL-60 cell line is a human leukemia cell line that has been used in laboratory studies of blood cell formation and physiology. The cell line was derived from a 36-year-old woman initially reported to have acute promyelocytic leukemia at MD Anderson Cancer Center. HL-60 cells exhibit predominantly neutrophil-promyelocytic morphology. This cell line can be induced to differentiate into mature granulocytes using compounds such as dimethyl sulfoxide (DMSO) or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 to differentiate to monocytic, macrophage-like and eosinophil phenotypes, respectively. Because of HL-60 cell's ability to differentiate into mature leukocytes and mimic the innate immune response, it has become an important model in cancer research, especially in the study of hematological malignancies, helping to understand leukemia progression, cellular oncogene expression, and identification of therapeutic targets. |
| Tissue | Peripheral blood |
| Disease | Acute promyelocytic leukemia |
| Morphology | Lymphoblast-like |
| Gender | Female |
| Age | 36 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 3D cell culture Immune system disorder research Cancer research Differentiation studies Immunology research Drug discovery and development |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the influence of physiological, pharmacological, and virological factors on this process. The HL-60 cell model is used to study the effects of DNA topoisomerase (topo) IIα and IIβ on cell differentiation and apoptosis, and is particularly suitable for dielectrophoresis research, because dielectrophoresis research requires a water environment for suspended and round cells. Additionally, these cells have been used to study whether intracellular calcium plays a role in reactive oxygen species-induced caspase activation. |
| Characteristics | |
| Karyotype | The number of chromosomes in the stem line is pseudodiploid, and the occurrence rate of 2S component is 6.2%. Most S metaphases share five markers (M2 to M6). DM has a different number of cells per cell and occurs in metaphase of all karyotypes. No HSR chromosome was detected. |
| Oncogene | myc+ |
| Tumorigenic | Yes, forms tumors in nude mice and semi-solid media. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. When starting from frozen ampoules, add thawed cells to conical centrifuge tubes e.g. 15 ml tube. 2. Slowly add 4 ml of medium to the tube. Take a sample of the cell suspension, e.g. 100 ul, for cell counting. 3. Centrifuge the cell suspension at low speed, i.e. 100-150 x g, for up to 5 minutes. 4. Remove the medium and resuspend the cell pellet in fresh medium containing 20% serum at a density of 3 - 5 x 100,000 cells/ml. 5. Incubate flask at 37℃; 5% CO2. Cells grow slowly after recovery and may take up to 10 days to establish proliferation. 6. Check daily. Once culture is established, serum concentration can be reduced to 10%. Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37℃; low density or they may differentiate. |
| Medium Renewal | Every 2 to 3 days |
| Interval | Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. |
| Culture Medium | RPMI 1640 + 2 mM Glutamine + 10-20% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃ |
| Cryopreservation | Frozen with 70% medium, 20% FBS, 10% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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