A549 Cell Line

Brief Introdution
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	A549 cells are adenocarcinoma human alveolar basal epithelial cells, a cell line first developed by D. J. Giard et al. in 1972. Cancerous lung tissue was removed and cultured from a transplanted tumor in a 58-year-old white man. These cells are used as models to study lung cancer and develop drug therapies against it.
Tissue	Lung
Disease	Carcinoma
Morphology	Epithelial-like
Gender	Male
Age	58 years
Product Format	Frozen
Growth Mode	Adherent or attaching to the culture flask
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	3D cell culture Bioproduction Cancer research High-throughput screening Toxicology
Shipped in	dry ice
Storage Temperature	−196°C
Additional Info	Studies by M. Lieber, et al. have shown that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway.
Characteristics
Virus Resistance	The cells are positive for keratin by immunoperoxidase staining.
Mycoplasma Test	Negative
Cytogenetic Information	The A549 cell line is hypotriploid with a chromosome mode of 66, present in 24% of cells. Modal numbers of 64 and 67 is relatively common, with higher ploidies occurring less frequently (0.4%). Immunoperoxidase staining was positive for A549 cytokeratin. These cells are capable of synthesizing lecithin and contain a high proportion of unsaturated fatty acids, which are utilized by the cytidine-diphosphocholine pathway and are important for maintaining intracellular membrane phospholipids.
Culture Conditions and Handling
Subculturing	1. Remove and discard culture medium. 2. Rinse the cell layer briefly with calcium- and magnesium-free PBS to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for the cells to detach. Cells that are difficult to separate can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and pipet gently to aspirate the cells. 5. Add appropriate aliquots of cell suspension to new culture vessels. 6. Incubate the culture at 37°C. The population doubling time is about 22 hours. NOTE: The volumes used in this protocol are for a 75 cm² flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium.
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	The ratio of 1:3 to 1:8 is recommended.
Culture Medium	Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate and fetal bovine serum to a final concentration of 10%.
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C
Cryopreservation	95% FBS + 5% DMSO (Dimethyl sulfoxide)

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Cat.No.	Product Name	Price
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* For research use only. Not intended for any clinical use.

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