Py230 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	Py230 is a murine mammary intraluminal tumor cell line with epithelial-like characteristics. It is derived from spontaneously generated mammary adenocarcinoma in female MMTV-PyMT (mutine mammary tumor virus promoter-driven polyomavirus T antigen) transgenic C57BL/6 mice. This cell line exhibits characteristics similar to normal murine mammary stem cells and is renowned for its lineage plasticity, meaning it can differentiate into intraluminal cells, myoepithelial cells, and alveolar cells upon stimulation. Py230 is an important model for studying the biology, tumor progression, and immune microenvironment of triple-negative breast cancer (TNBC).
Tissue	Mammary gland (Breast)
Disease	Mammary Gland Malignant Neoplasm (Mammary adenocarcinoma)
Morphology	Epithelial-like; cuboidal-shaped cells that grow in well-differentiated colonies at confluence
Gender	Female
Age	Adult
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Triple-negative breast cancer (TNBC) research 2. Study of mammary tumorigenesis and metastatic behavior 3. Investigation of cell state transitions and lineage plasticity 4. Preclinical testing for breast cancer therapeutic strategies 5. Analysis of tumor-immune microenvironment and immunosuppression
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Karyotype	Aneuploid (specifically in certain sublines with dysregulated p53); Generally noted for its genetic stability in specific research contexts
Tumorigenic	Yes; forms luminal-type primary tumors and lung metastases in syngeneic or immunocompromised mice
Expression Markers	Positive for luminal-epithelial markers E-cadherin, Cytokeratin 8, and Cytokeratin 14; Negative for Estrogen Receptor (ER) and Progesterone Receptor (PR) in vitro; Low levels of HER2
Protein Expression	Polyoma virus middle T oncogene; p53 (can be nuclear positive in mesenchymal-like sublines)
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Rinse the cell layer with PBS or cell dissociation buffer to remove all serum residue. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution and observe under a microscope until the cell layer disperses (usually 5 to 15 minutes). Note: To avoid cell clumping, do not tap or shake the culture flask during cell dissociation. 4. Add 6.0 to 8.0 mL of complete culture medium and gently aspirate the cells using a pipette. 5. Aliquot the cell suspension into new culture containers; a seeding density of 1 × 10^4 to 5 × 10^4 viable cells/cm² is recommended.
Medium Renewal	Every other day or 2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:3 to 1:6 is recommended
Culture Medium	F-12K Medium supplemented with 5% Fetal Bovine Serum (FBS); Some protocols recommend adding MITO+ Serum Extender to maintain differentiation properties.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% to 10% (v/v) DMSO

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

Cat.No.	Product Name	Price
CSC-RO01216	Cas9 Stable Cell Line - Py230	Inquiry
CSC-RR00821	Luciferase Reporter Cell Line - Py230	Inquiry
CSC-RR00822	GFP Reporter Cell Line - Py230	Inquiry

* For research use only. Not intended for any clinical use.

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