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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | HCC827 is a human non-small cell lung cancer cell line (NSCLC) derived from a lung adenocarcinoma in a middle-aged female patient. These cells exhibit epithelial morphology and are commonly used in studies related to the epidermal growth factor receptor (EGFR). The HCC827 NSCLC-derived xenograft model contains an adenocarcinoma cell line that harbors an activating EGFR mutation in exon 19. This mutation occurs in approximately 48% of EGFR-mutant NSCLC cases and confers increased sensitivity to EGFR tyrosine kinase inhibitors (TKIs), which has been validated in HCC827. The model also exhibits high expression of PD-L1, which may contribute to cancer immune escape. These properties of HCC827 make it a relevant model for preclinical studies of NSCLC. The cell line is also used to explore targeted therapeutic mechanisms of acquired resistance, a major challenge in lung cancer treatment. |
| Tissue | Lung |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 39 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. Cancer research 2. Drug screening and sensitivity studies 3. Study on drug resistance mechanism 4. Genetic studies 5. Signal transduction research 6. In vitro and in vivo models |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | This lung adenocarcinoma harbors an acquired mutation in the EGFR tyrosine kinase domain (deletion E746 - A750). HCC827 cells are particularly sensitive to tyrosine kinase inhibitors (TKIs), especially those targeting EGFR mutations. This property makes them a valuable model for studying the molecular mechanisms of lung cancer response to EGFR inhibitors and for testing the efficacy of new therapeutic agents targeting EGFR-dependent pathways. |
| Characteristics | |
| Tumorigenic | Yes, in nude mice. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | The volumes used in this protocol are for 75 cm2 flasks. For culture vessels of other sizes, the amount of dissociation medium should be proportionally reduced or increased. 1. Remove and discard the medium. 2. Briefly rinse the cell layer with 0.25% (w/v) trypsin - 0.53 mM EDTA solution or Dulbecco's phosphate-buffered saline (D-PBS) to remove all traces of serum containing trypsin inhibitors. 3. Add 1.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping, do not agitate the cells by tapping or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach can be placed at 37°C to facilitate dispersion. 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to aspirate the cells. 5. Transfer the cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. 6. Discard the supernatant and resuspend the cells in fresh growth medium. Add the appropriate cell suspension to a new culture vessel. The recommended inoculum size is 5 x 103 to 7 x 103 viable cells/cm2. 7. Place the culture vessel in a 37°C incubator. Maintain the culture at a cell concentration between 3 x 104 and 5 x 104 cells/cm2. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | The ratio of 1:4 to 1:6 is recommended. |
| Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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