Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Recombinant adenovirus, a widely used gene delivery vector, boasts advantages such as large packaging capacity, high titers, and efficient transgene expression in various cell types. Unlike integrating viruses, adenoviruses don't integrate into the host genome, ensuring operational security. Creative Biogene provides premade adenovirus particles, including control adenoviruses with GFP, RFP, and luciferase. These control adenoviruses serve as transduction controls during target cell infection. Additionally, customization options include fifty cell-specific promoters and fluorescent reporters. Explore our products or contact us for specific requirements.
Benefits of Our Control Adenovirus Particles
Control Adenovirus Particles Product List
Creative Biogene's control adenovirus panel facilitates optimal viral dose identification for efficient target cell transduction. Quantifying reporter expression levels enables MOI determination with the highest gene delivery and minimal toxicity. The principle involves transduction with matched-titer control viruses for infectivity comparison, aiding parameter optimization like incubation time. Standardized Creative Biogene control adenoviruses offer convenient tools for determining effective infection conditions across the following diverse applications:
Case Study 1
In the context of liver transplantation, acute rejection poses a significant threat to patient prognosis. The researchers investigate the role of miR-505-5p in liver transplantation acute rejection. Using an allogeneic rat liver transplantation model, they analyze morphological changes, and assess Myd88, miR-505-5p, IL-10, and TNF-α expression, confirming Myd88 as a direct target of miR-505. MiR-505-5p's role in alleviating rejection suggests a potential post-transplantation treatment strategy based on miRNAs.
Figure 1. Cells were transfected with Myd88 adenovirus (adv-Myd88) or control adenovirus (adv-NC). (Chai H, et al., 2022)
Case Study 2
Abdominal aortic aneurysm (AAA) is characterized by aorta dilation due to wall degeneration, which mostly occurs in elderly males. Researchers found that elevated PDE1C in abdominal aortic aneurysms links to vascular smooth muscle cell aging, presenting a potential therapeutic target. In this study, SIRT1-mediated regulation of smooth muscle cell (SMC) aging was investigated using SIRT1 inhibitors (EX-527 and (S)-35) in SMCs derived from PDE1C or PDE1C knockout mice, and adenoviral transfection was employed to modulate PDE1C expression.
Figure 2. SIRT1 protein expression was assessed in PDE1C knockout smooth muscle cells (SMCs) through transfection with adenovirus Ad-EGFP (control) or Ad-mPDE1C1-Flag (Ad-PDE1C1) to induce PDE1C overexpression. (Zhang C, et al., 2021)
Case Study 3
QCR2, crucial for mitochondrial function, eludes a clear understanding of cancer development. Researchers immunohistochemically assess QCR2 expression in cancer patients. Proliferation was evaluated via CCK-8, staining, and flow cytometry. The biological functions of QCR2 and PHB were examined through protein blotting, RT-qPCR, microarray, and xenografts. Protein interactions and p53 ubiquitination were assessed via immunoprecipitation, mass spectrometry, and GST pull-down. Subcellular locations of PHB and QCR2 were examined through immunoblotting and immunofluorescence. In the study, control adenovirus refers to adenovirus carrying an empty pcDNA vector control, serving as a baseline control in the experimental design. Meanwhile, QCR2-Flag Ad represents adenovirus containing a vector with QCR2-Flag, allowing for the overexpression of QCR2.
Figure 3. Adenoviral-mediated QCR2-Flag overexpression led to p53 and p21 downregulation, implying QCR2 inhibition of p53 activity. Conversely, QCR2 knockdown had no impact on p53 and p21 levels in p53R273C-mutant C33A cervical cancer cells. (Han Y, et al., 2019)
Q: What is the main purpose of Control Adenovirus Particles in experiments?
A: Control Adenovirus Particles act as a baseline group, allowing researchers to isolate and understand the effects caused by a specific transgene by eliminating the influence of non-specific adenovirus responses.
Q: In which experimental scenarios are Control Adenovirus Particles particularly valuable?
A: Control Adenovirus Particles are valuable when researchers aim to attribute observed effects to the introduced transgene. They provide a reference point for comparison to isolate specific transgene-related impacts.
Q: How can researchers ensure the specificity of observed effects when using Control Adenovirus Particles?
A: Researchers can perform specificity testing by comparing effects induced by Control Adenovirus Particles with those carrying a transgene. Any effects seen in the control group are subtracted from the total effects observed in experimental groups.
Q: Are there considerations or precautions for researchers using Control Adenovirus Particles?
A: Researchers should be mindful of potential non-specific adenovirus responses, carefully choose an appropriate control group, and design experiments rigorously for accurate conclusions. It's crucial to ensure minimal effects in the control group, avoiding confounding factors that could hinder the interpretation of experimental outcomes.
| Cat.No. | Product Name | Price |
|---|---|---|
| AD00007Z | LacZ adenoviral particles | Inquiry |
| AD00281Z | GFP adenoviral particles | Inquiry |
| AD00301Z | 6xHis adenovirus | Inquiry |
| AD00303Z | Adenovirus-Null | Inquiry |
| AD00304Z | Adenovirus-Syn-Null | Inquiry |
| AD00330Z | Syn-GFP adenovirus | Inquiry |
| AD00332Z | EYFP-Actin adenovirus | Inquiry |
| AD00335Z | Luc adenovirus | Inquiry |
| AD00336Z | FLAG adenovirus | Inquiry |
| AD00357Z | Syn-mCherry adenovirus | Inquiry |
| AD00358Z | mCherry adenovirus | Inquiry |
| AD00360Z | mRFP adenovirus | Inquiry |
| AD00376Z | Syn-RFP adenovirus | Inquiry |
| AD00384Z | U6-shRNA-GFP adenovirus | Inquiry |
| AD00385Z | U6-shRNA-RFP adenovirus | Inquiry |
Copyright © Creative Biogene. All rights reserved.