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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | BCPAP is a human thyroid cancer cell line isolated in 1992 from the metastatic papillary carcinoma tissue of a 76-year-old female patient. BCPAP is currently one of the most widely used models for investigating the biological characteristics of thyroid cancer. BCPAP cells are characterized by the presence of a homozygous BRAF V600E mutation, which results in the constitutive activation of the MAPK/ERK signaling pathway. This cell line exhibits an epithelial morphology and is renowned for its high invasiveness and metastatic potential. It serves as an indispensable tool for studying the molecular pathogenesis of papillary thyroid carcinoma, the mechanisms of radioiodine resistance, and the development of targeted kinase inhibitors. |
| Tissue | Thyroid |
| Disease | Papillary Thyroid Carcinoma (PTC) |
| Morphology | Epithelial |
| Gender | Female |
| Age | 76 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Papillary thyroid carcinoma (PTC) pathogenesis and metastasis research 2. Study of BRAF V600E-mediated MAPK/ERK signaling 3. Evaluation of novel BRAF and MEK inhibitors 4. Investigation of sodium iodide symporter (NIS) regulation and radioiodine therapy resistance 5. Development of human tumor xenograft models in nude mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in immunocompromised (nude) mice |
| Karyotype | Aneuploid; hypertriploid |
| Genetic Profile | BRAF mutation (V600E, homozygous); TP53 mutation (D259Y); hTERT promoter mutation |
| Expression Markers | Positive for thyroid-specific markers (e.g., PAX8) but often shows reduced expression of differentiation markers like TG and NIS |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with PBS (without Ca2+/Mg2+) to remove all traces of serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin - 0.53 mM EDTA solution and observe under an inverted microscope until the cell layer is dispersed (usually 5 to 12 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium and gently pipette to obtain a single-cell suspension. 5. Aliquot cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00737 | Luciferase Reporter Cell Line - Bcpap | Inquiry |
| CSC-RR00982 | GFP/Luc Reporter Cell Line - B-cpap | Inquiry |
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