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Agrobacterium-mediated Transformation Service

Creative Biogene is engaged in providing high quality Agrobacterium-mediated transformation service to our clients. With years of experience and mature experimental systems in plant genetic engineering, along with our state-of-the-art facilities, scientists at Creative Biogene develop an excellent transformation platform.

Agrobacterium tumefaciens, a soil phytopathogen, naturally infects plant wound sites and causes crown gall disease via delivery of transferred (T)-DNA from bacterial cells into host plant cells. With this characteristic, researchers accomplish the integration of plant genome by inserting foreign DNA fragments into T-DNA. Comparing with other delivery strategies, Agrobacterium-mediated transformation shows many advantages, such as low copy numbers integration, less expensive, high efficiency, stable integration and minimal DNA rearrangements. Currently, Agrobacterium-mediated transformation is the most common method of genetic engineering in plants, including most dicots and a few monocots. The transformation efficiency of many plants can reach up to 50%.

The Mechanism of Agrobacterium-mediated TransformationFigure 1. The Mechanism of Agrobacterium-mediated Transformation

Creative Biogene develops an efficient and versatile platform that offers transformation services for more than 30 plant species covering model plants, grain crops and cash crops. We provide various Agrobacterium strains such as GV3101, AGL-1, EHA101, EHA105, LBA4404, C58C1, and selection markers such as kanamycin, hygromycin, phosphinothricin, glufosinate, for different receptor species and research purposes.

Advantage

  • High efficiency: reach up to 50%-80%.
  • Short turnaround time: about 6 to 9 months.
  • Various genotype: about 5 genotypes for common species.
  • Affordable price: base on the scope of the project and numbers of independent events.

Work flow

Agrobacterium-mediated Transformation Service

This process applies to common plants, such as crops and model plants. We also work with you to develop custom transformation system for some rare plants to meet your research requirements.

For more information, please contact us at or 1-631-626-9181. We are waiting for your interest projects.

Reference:
  1. Newell, C. A. (2000). Plant transformation technology. Molecular Biotechnology, 16(1), 53-65.

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