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Fig.1 Schematic overview of delivery of
IVT mRNA into cells. [2]
Messenger RNA (mRNA), a natural biomolecule, is a transient entity that mediates the translation of genetic information from genes encoded in DNA to proteins located throughout the cell. mRNA has recently emerged with remarkable potential as a gene delivery molecule in the fields of inherited genetic disorders, cancer immunotherapy and stem cell-based biomedical research as an alternative to plasmid DNA. As a direct source of gene products, mRNA has several advantages: It does not require nuclear entry for transfection activity and has a negligible chance of integrating into the host genome which excludes the possibility of potentially detrimental genomic alternations. Chemical modification of mRNA has further enhanced its stability and decreased its activation of innate immune responses. Additionally, mRNA has been found to have rapid expression and predictable kinetics. However, given the unfavorable properties of mRNAs, such as large size, negative charge, their uptake to cells is prevented. Thus, an efficient IVT mRNA delivery system is required to deliver synthetic mRNA into cells.
Creative Biogene, as one of the leading biotechnology companies, can provide high-quality transfection reagents for customers worldwide to help you deliver mRNA in a variety of cells or in vivo.
References:
| Cat.No. | Product Name | Price |
|---|---|---|
| RNTK-001 | mRNA in vitro transfection kit | Inquiry |
| RNTK-002 | mRNA in vivo delivery kit | Inquiry |
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