Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | PANC-1 is a human pancreatic cancer cell line isolated from ductal cell-derived pancreatic cancer. PANC-1 was derived from tissue from a 56-year-old man. Cells can transfer but have poor differentiation ability. PANC-1 cells require 52 hours for population doubling, have a chromosome mode of 63, and display slow-migrating G6PD. |
| Tissue | Pancreas |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Male |
| Age | 56 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 3D cell culture Cancer research |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | PANC-1 cells have been used to study the role of keratin reorganization during the migration of cancer cells, along with calcium-mediated actin reset in response to physiological changes. |
| Characteristics | |
| Protein Expression | p53 positive, CEA negative |
| Isoenzymes | G6PD, B |
| Tumorigenic | Growth in soft agar. Formation of progressively growing carcinomas in nude athymic mice. |
| Cytogenetic Information | The karyotype information of PANC-1 cells shows that they are hyperdiploid with a chromosome mode of 63. Interestingly, these cells had lost their Y chromosome, a common phenomenon in cancer cell lines due to genetic instability. Key protein markers expressed by PANC-1 cells include CK5.6, MNF-116, vimentin, chromogranin A, CD56, and SSTR2. |
| Culture Conditions and Handling | |
| Thawing Frozen Cells | 1. Thaw vials in a 37°C water bath with gentle stirring. To reduce the possibility of contamination, keep the Oring and cap out of the water. Thawing should be quick (about 2 minutes). 2. Immediately after the contents have thawed, remove the vial from the water bath and decontaminate it by immersing or spraying it in 70% ethanol. All operations thereafter should be performed under strict sterile conditions. 3. For cells that are sensitive to DMSO, it is recommended to remove the cryoprotectant immediately. Transfer the contents of the vial to a centrifuge tube containing 9.0 mL of complete medium and spin at approximately 125 x g for 5 to 7 minutes. 4. Discard the supernatant and resuspend the cell pellet in the recommended complete medium. 5. Incubate the culture in the appropriate atmosphere and temperature. |
| Subculturing | Remove culture medium and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Leave the flask at room temperature (or 37°C) until cells detach. Add fresh medium, blot dry and dispense into new culture flasks. Corning T-75 flasks are recommended for passage of this product. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A ratio of 1:2 to 1:4 is recommended |
| Culture Medium | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
The above is only part of a part of cell line products. If you don’t find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO01233 | Cas9 Stable Cell Line - PANC-1 | Inquiry |
| CSC-RO1044 | Human CLDN18-A2 Stable Cell Line-Panc-1 | Inquiry |
| CSC-RR00626 | Luciferase Reporter Cell Line - PANC-1 | Inquiry |
| CSC-RR0357 | GFP Reporter Cell Line - PANC-1 | Inquiry |
| CSC-RR0488 | GFP/Luc Reporter Cell Line - PANC-1 | Inquiry |
| CSC-RT2579 | Human CAV2 Knockout Cell Line-panc-1 | Inquiry |
| CSC-RT2664 | Human PTEN Knockout Cell Line-Panc-1 | Inquiry |
| CSC-RT2709 | Human FAS Knockout Cell Line-PANC-1 | Inquiry |
| CSC-RT2820 | Human GPC1 Knockout Cell Line-PANC1 | Inquiry |
Copyright © Creative Biogene. All rights reserved.