Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
A cloning vector is a small DNA fragment derived from a virus, plasmid, or higher organism cell, which can be stably maintained in an organism and serves as a vehicle for the insertion of foreign DNA fragments for cloning purposes. These foreign DNA fragments are replicated and maintained as the host organism undergoes division and growth, resulting in a clonal population. Cloning vectors provide the fundamental framework for the replication of DNA inserts but are solely utilized for cloning and not for expressing protein products. DNA inserts within cloning vectors can be replicated but not translated into functional protein products. As an essential tool for the successful cloning of foreign DNA fragments, Creative Biogene offers a comprehensive collection of cloning vectors. Please browse through our series of cloning vectors to find the one that suits your needs.
Key Features of Our Cloning Vector
In the fields of molecular biology and genetic engineering, cloning vectors serve as crucial tools for the insertion, amplification, and transmission of DNA fragments. These vectors play essential roles in gene cloning, DNA sequence analysis, gene expression, and gene editing. The listed vectors, such as pUC, pBluescript, and pACYC, are common plasmid vectors used for DNA cloning in bacteria such as Escherichia coli. Other vectors, including pMD18-T, pMD19-T, and pGEM-T easy, feature T-tailed structures enabling direct cloning of PCR products. Additionally, vectors like pDONR207 and pDONR221 contain the Gateway recombination sites, allowing rapid, efficient transfer of sequences into a variety of expression systems using site-specific recombination.
Case Study 1
During co-evolution with pathogens, plants have developed effector-triggered immunity (ETI) as a defense mechanism against plant pathogenic bacteria, which inject effectors into plant cells via type III secretion systems (T3SS) to evade the plant's immune system and enhance infection. By screening 6,200 transposon-tagged mutants of the bacterial pathogen Acidovorax avenae strain N1141, they identified 17 mutants lacking the ability to induce hypersensitive response (HR) cell death in rice. Through sequence analysis and T3SS-mediated intracellular transport, they identified a candidate effector protein called rice HR cell death-inducing factor (RHIF). Disruption of RHIF in N1141 abolished its ability to induce HR cell death, while complementation with RHIF restored this ability. Additionally, RHIF from the virulent strain K1 induced ETI in the non-host plant finger millet. These findings suggest that RHIFs play crucial roles in host plant infection and ETI induction in non-host plants, shedding light on the mechanisms underlying plant immunity and pathogen virulence.
Figure 1. The pBBR1Tp vector was used by researchers to express the NT5 (RHIF)-CyaA fusion protein, enabling its transport from N1141 into rice cells. This allowed for the measurement of cAMP concentration, providing insights into the function of NT5 (RHIF). (Nakamura M, et al., 2021)
Case Study 2
Voltage-dependent L-type Ca2+ (CaV1.2) channels are the key Ca2+ entry pathway in arterial myocytes, regulating vascular functions and implicated in diseases like hypertension. However, the molecular identity of these channels in myogenic arteries, crucial for blood pressure and flow regulation, remains unknown. Researchers utilized the pGEM-T vector to clone CaV1.2 subunits from resistance-size cerebral arteries, aiming to identify the molecular identity of CaV1.2 channels expressed in myocytes of myogenic arteries regulating vascular pressure and blood flow. Through quantitative PCR and functional assays, they discovered a novel exon 1c-derived N terminus, which exhibited distinct voltage-dependent Ca2+ entry characteristics in arterial myocytes, indicating tissue-specific Ca2+ signaling regulation.
Figure 2. Full-length CaV1.2 cDNAs cloned from rat small cerebral arteries were subcloned into the pGEM-T Easy vector by researchers for further manipulation and analysis, facilitating downstream experiments. (Cheng X, et al., 2007)
Case Study 3
Researchers have identified a mechanism of gene amplification in Escherichia coli and Klebsiella pneumoniae, resulting in resistance to piperacillin/tazobactam (TZP) while remaining susceptible to carbapenems and 3rd generation cephalosporins. This involves hyperproduction of the β-lactamase TEM, facilitated by a translocatable unit (TU) excising from an IS26-flanked pseudo-compound transposon, PTn6762. The TU re-inserts into the chromosome adjacent to IS26, forming a tandem array of TUs, increasing blaTEM-1B copy number and leading to TEM-1B hyperproduction and TZP resistance, without incurring a fitness cost compared to TZP-susceptible strains. Understanding this gene amplification mechanism is crucial for predicting TZP susceptibility using genomic data.
Figure 3. Using the CHL-resistant pUC vector, pHSG396, containing a copy of IS26 from the TZP-susceptible chromosome, the evolutionary event was replicated, and the excised TU adjacent to the IS26 copy in the plasmid was captured by researchers, providing evidence of the insertion event. However, no evidence of tandem repeats of the TU in the pHSG396:IS26 plasmid was found. (Hubbard ATM, et al., 2020)
Q: Which cloning vector should I choose?
A: Creative Biogene's cloning vectors offer a variety of options, covering common plasmid vectors such as pUC, pBluescript, etc., as well as specially designed vectors like pGEM-T easy, pDONR221, etc. You can choose the most suitable vector based on your experimental requirements and desired cloning strategies.
Q: What are the features of these cloning vectors?
A: Our cloning vectors possess stable DNA replication capability, selection markers and other basic components to ensure accurate insertion, replication, and transmission of your DNA fragments.
Q: How is the performance of these cloning vectors?
A: Our cloning vectors undergo rigorous quality control and validation to ensure their stability and reliability. Creative Biogene’s cloning vectors have been widely applied in molecular biology and genetic engineering fields, gaining recognition and trust from many researchers.
Q: What are the advantages of your cloning vectors?
A: Our cloning vectors are highly efficient in gene transfer, allowing for the production of multiple copies of the target gene. Moreover, they are highly adaptable and can be designed to fit specific cloning needs.
Q: Can I obtain customized cloning vectors?
A: Yes, we provide customized services to tailor cloning vectors according to your specific needs. If you require specific vector designs or functional modules, our team will be dedicated to providing support and customized solutions.
| Cat.No. | Product Name | Price |
|---|---|---|
| VCT1105 | pACYC177 vector | Inquiry |
| VCT1106 | pACYC184 vector | Inquiry |
| VCT1107 | pACYCDuet-1 | Inquiry |
| VCT1108 | pBBR1Tp vector | Inquiry |
| VCT1109 | pRS316 vector | Inquiry |
| VCT1110 | pUC18 vector | Inquiry |
| VCT1111 | pUC19 vector | Inquiry |
| VCT1112 | pUC57 vector | Inquiry |
| VCT1113 | pUC118 vector | Inquiry |
| VCT1114 | pUC119 vector | Inquiry |
| VCT1115 | pBluescript SK(+) vector | Inquiry |
| VCT1116 | pBluescript II SK(+) vector | Inquiry |
| VCT1117 | pBR322 vector | Inquiry |
| VCT1118 | pGEM-T easy vector | Inquiry |
| VCT1119 | pGEM®3Zf(+) vector | Inquiry |
Copyright © Creative Biogene. All rights reserved.