Daoy Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	The Daoy cell line, the first established human medulloblastoma cell line, was isolated in 1985 from a posterior fossa tumor in a 4-year-old Caucasian male. Medulloblastoma is a highly malignant primary brain tumor originating in the cerebellum and is the most common brain cancer in children. Daoy cells exhibit an adherent growth pattern and epithelioid morphology. This cell line is a fundamental model in neuro-oncology for studying the molecular pathogenesis of Sonic Hedgehog (SHH) subgroup medulloblastoma. It is widely used to study tumor cell migration, invasion, and the efficacy of small molecule inhibitors and radiotherapy for central nervous system tumors in children.
Tissue	Brain; Cerebellum
Disease	Medulloblastoma; Desmoplastic cerebellar medulloblastoma
Morphology	Epithelial-like; polygonal
Gender	Male
Age	4 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	1. Pediatric neuro-oncology and medulloblastoma research 2. Study of Sonic Hedgehog (SHH) signaling pathway 3. Investigation of brain tumor cell invasion and migration 4. Screening for novel pediatric brain cancer therapeutics 5. Evaluation of radiobiology and radiation resistance mechanisms
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Karyotype	Hypotriploid; Modal number = 58; range = 55 to 60
Tumorigenic	Yes, in immunocompromised (nude) mice
Genetic Profile	TP53 mutation; MYC amplified in some sublines; SHH subgroup characteristics
Protein Expression	Positive for neuron-specific enolase (NSE) and synaptophysin
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Quickly wash the cell layer with Ca2+/Mg2+-free PBS buffer to remove all serum residue. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution and observe under an inverted microscope until the cell layer disperses (usually 5 to 10 minutes). 4. Add 6.0 to 8.0 mL of complete culture medium and gently pipette until the cell suspension is a single-cell suspension. 5. Aliquot the cell suspension into new culture dishes.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:3 to 1:8 is recommended
Culture Medium	Eagle's Minimum Essential Medium (EMEM) or DMEM/F12 supplemented with 10% Fetal Bovine Serum (FBS).
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% to 10% (v/v) DMSO

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Cat.No.	Product Name	Price
CSC-RR00753	GFP Reporter Cell Line - Daoy	Inquiry
CSC-RR00754	Luciferase Reporter Cell Line - Daoy	Inquiry

* For research use only. Not intended for any clinical use.

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