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Yeast Expression System (Pichia pastoris)

Yeast expression system is the most economical eukaryotic expression system for both secreted and intracellular proteins. Yeast expression system possesses the advantages of both prokaryotic and eukaryotic expression systems, such as high expression level, low cost, easy to scale up and proper protein folding which make it suitable for both small and large scale production of interested proteins. Creative Biogene as a leading biotechnology company possesses advanced technologies and expertise experience which can offer the complete protein production and purification services to customers worldwide. Creative Biogene’s goal is to provide you with the most affordable and high-quality gene expression service based on Yeast expression systems (Pichia pastoris) to ensure your satisfaction in a timely and professional manner.

Pichia pastoris is methylotrophic yeast that is frequently used as an expression system for the production of foreign proteins. Pichia pastoris as a single-celled microorganism is easy to manipulate and culture which makes it easier to use, and more cost-efficient than other eukaryotic expression systems, e.g. mammalian expression systems. Besides, as a eukaryote, it possesses the capabilities of post-translational modifications performed by higher eukaryotic cells. Proteins that express as inactive inclusion bodies in E.coli expression systems can therefore be produced as biologically active molecules in Pichia pastoris. As yeast, it shares the advantages of easy genetic manipulations similar with Saccharomyces cerevisiae, moreover, it can grow to very high cell densities which make it possesses the advantage of 10-100 folds higher heterologous protein expression levels. All the above features have made Pichia pastoris become a very useful protein expression system for both researchers and industry.

Applications:

  • Eukaryotic protein analysis, e.g. structural studies
  • Research tool proteins for crystallography
  • Large-scale protein production for feed studies
  • Production of isotope labeled protein for NMR

Features:

  • One-stop solution from gene synthesis/gene cloning to purified protein
  • High Efficiency: various strains and expression vectors available to select for the most suitable expression host and vector
  • High Productivity: robust secretion signal sequences to ensure the highest secretion of target protein
  • Short timeline from gene synthesis/cloning to protein recovery
  • Cost-saving: provide high quality proteins with competitive price

Creative Biogene provides gene expression service based on Pichia pastoris expression system, which includes:

  • Codon optimization for any given expression system (upon request)
  • Gene synthesis, subclone gene of interest into yeast expression vector
  • Linearized vector and electricity transforming into Pichia pastoris
  • Screening and strain creation
  • Flexible scale protein expression and purification
  • Protein post processing (optional) such as purified protein tag removal, filtration sterilization, endotoxin removal, freeze-dry, N-terminal sequencing et al.)

References:

  1. Higgins DR. (2001). Overview of protein expression in Pichia pastoris. Current protocols in protein science, 5-7.
  2. Cregg JM. (2007). Introduction: distinctions between Pichia pastoris and other expression systems (pp. 1-10). Humana Press.
  3. Lundblad RL. (1999) Glycosylation in Pichia pastoris. Biotechnology and applied biochemistry, 30(3): 191-192.

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