Yeast Three-hybrid cDNA Library Construction Service

Creative Biogene is one of the leading biotechnology companies which can provide high quality yeast three-hybrid cDNA library construction services for customers worldwide. Creative Biogene state-of-the-art facilities and highly experienced staffs are available to assist you in all aspects of total RNA extraction, reverse transcription, library construction and qualification. With years of experience in the cDNA library construction field, Creative Biogene has confident to provide you with high-quality yeast three-hybrid cDNA library construction services to ensure your satisfaction in a timely and professional manner.

Fig.1 General strategy of the Yeast three-hybrid system.[2]

Yeast three-hybrid system is a derivative of yeast two-hybrid (Y2H) and it is a kind of powerful tool to detect RNA-protein interactions. In this system, a transcription factor is assembled by the use of an RNA bridge that brings a fusion protein containing a DNA-binding domain together with a fusion protein containing an activation domain. The assembly of this ternary complex usually depends on each fusion protein containing an RNA-binding domain that binds to a site in the RNA molecule. When this ternary complex forms at a promoter, even transiently, the expression of the reporter gene is turned on. Reporter expression can be detected by cell growth, colony color, or the levels of a specific enzyme. Thus, the RNA-protein interactions can be monitored by phenotype or simple biochemical assays.

Applications:

Discovery of proteins that bind to a known RNA sequence
Identification of RNA sequences that bind known RNA-binding proteins
Testing suspected interactions between an RNA and protein
Mutational analysis of interacting RNAs and proteins
Discovery and analysis of multiprotein–RNA complexes

Creative Biogene offers yeast three-hybrid cDNA library services, which can accelerate your RNA-protein interaction researches. Our experienced experts' team along with our broad services portfolio makes it easier to meet the demands of different researchers. Dedicated scientists in Creative Biogene who have years of experience in cDNA library construction will offer you the most affordable, high-quality yeast three-hybrid cDNA library services in a short turnaround time. If you are interested in our services, please do not hesitated to contact us. We are looking forward to your attractive projects.

References:

Stumpf CR, et al. (2008). Analysis of RNA–protein interactions using a yeast three-hybrid system. Methods in enzymology, 449, 295-315.
Bernstein DS, et al. (2002). Analyzing mRNA–protein complexes using a yeast three-hybrid system. Methods, 26(2), 123-141.
SENGUPTA DJ, et al. (1999). Identification of RNAs that bind to a specific protein using the yeast three-hybrid system. Rna, 5(4), 596-601.

* For research use only. Not intended for any clinical use.

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