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Stable Knock-in Cell Line Generation

Brief Introduction


CRISPR/Cas9-induced site-specific DNA double-strand breaks (DSBs) can be repaired by homology-directed repair (HDR) or non-homologous end joining (NHEJ) pathways. Extensive efforts have been made to knock-in exogenous DNA to a specific genomic locus via the HDR pathway in mammalian cells.

Creative Biogene, as a leader in cell engineering, provides reliable, affordable, and fast knock-in cell line generation service, which includes gRNA/donor DNA construction, PCR sequencing, and engineered cell line generation. We have experience working with a wide variety of cell backgrounds ranging from easy-to-transfect cell lines (like HEK2993, HCT116 or HeLa) to hard-to-transfect cell lines (like iPSCs, fibroblasts). Creative Biogene produces knock-in cell lines and performs quality control testing in one step to help ensure that the cell line meets your requirements.

Features

  1. One stop service- We provide full service from gRNA/donor DNA construction to engineered cell line generation.
  2. Reliable- All stable cell lines provided by Creative Biogene are guaranteed free of mycoplasma contamination.
  3. Custom services- We provide custom services tailor made based on your request.
  4. A wide variety of cell type- We have worked with everything from HEK293 to iPSCs.
  5. Kinds of genome modifications- Introduce/correct point mutations, safe harbor KI, SNP edit and insert a tag/fluorescent protein into the target gene.

Process

  1. gRNA/donor DNA construction
  2. Co-transfect into cells of interest
  3. Validation of custom CRISPR efficacy
  4. Screening of positive cell clones
  5. Quality control analysis of stable cell line
  6. Delivery of stable cell line

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