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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | MDA-MB-231 cell line, established in 1976 by J.W. Shay and colleagues, originated from a tumor obtained from a 51-year-old woman diagnosed with metastatic breast cancer. These cells exhibit a characteristic called epithelial-mesenchymal transition (EMT), which is associated with the ability of cancer cells to undergo metastatic proliferation and invade surrounding tissue. The MDA-MB-231 cell line is classified as a "triple-negative" breast cancer. This means that these cells do not express the estrogen receptor, progesterone receptor, or HER2 protein. |
| Tissue | Breast |
| Disease | Adenocarcinoma |
| Morphology | Epithelial |
| Gender | Female |
| Age | 51 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 3D cell culture Cancer biology study Drug screening Drug resistance study |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Additional Info | Triple-negative breast cancer is known for its aggressiveness and resistance to hormone therapy or treatments that target the HER2 protein. The lack of these receptors limits the available treatment options for this breast cancer subtype. The MDA-MB-231 cell line exhibits invasive behavior and is highly susceptible to metastasis, making it a valuable model for studying mechanisms and characteristics associated with metastatic breast cancer. |
| Characteristics | |
| Karyotype | This cell line is aneuploid female (mode = 64, range = 52 to 68) with chromosome counts approaching the triploid range. Normal chromosomes N8 and N15 are absent. In addition to most trisomic autosomes, eleven stably rearranged marker chromosomes as well as nonassignable chromosomes were noted. |
| Tumorigenic | Yes, in ALS treated BALB/c mice and nude mice, forms poorly differentiated adenocarcinoma (grade III). |
| Oncogene | wnt7b |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell layer with a 0.25% (w/v) trypsin-0.53 mM EDTA solution to remove all traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of trypsin-EDTA solution to the flask and observe the cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). 4. NOTE: To avoid clumping, do not agitate the cells by knocking or shaking the flask while waiting for cells to detach. Cells that are difficult to separate can be placed at 37℃ to facilitate dispersion. 5. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gentle pipetting. 6. Add appropriate aliquots of cell suspension to new culture vessels. 7. Cultures were grown at 37℃ in the absence of CO2. NOTE: The volumes used in this protocol are for a 75 cm2 flask. For other sizes of culture vessels, proportionally reduce or increase the amount of dissociation medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:4 is recommended. |
| Culture Medium | L15 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS). |
| Culture Conditions | Air: 100%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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