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| General Information | |
| Organism | Rat |
| Cell Line Description | HBZY-1 cells are a special biological resource derived from kidney glomeruli, specifically from a cell type called mesangial cells. These remarkable cells originate from Rattus norvegicus, commonly known as the rat. Glomeruli are an important part of the kidneys and are responsible for filtering and purifying blood. Mesangial cells within the glomerulus are critical for maintaining the structural integrity and function of this specialized nephron. In vitro studies using HBZY-1 cells allow researchers to study cellular processes, signaling pathways, and molecular interactions involved in mesangial cell biology. HBZY-1 cells provide many opportunities to study various aspects of kidney biology and advance our understanding of kidney-related diseases and conditions. |
| Tissue | Kidney |
| Morphology | Epithelial |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | 1. The HBZY-1 cell line can help researchers study cellular processes, signaling pathways, and molecular interactions involved in mesangial cell biology. 2. HBZY-1 cells are an excellent model for studying the pathophysiology of kidney diseases such as glomerulonephritis and diabetic nephropathy. By subjecting these cells to a variety of experimental conditions, researchers can model disease states and explore the underlying molecular events that lead to kidney pathology. 3. The emergence of HBZY-1 cells opens new avenues for drug discovery and therapeutic intervention in kidney-related diseases. These cells can screen potential therapeutic compounds, assess their efficacy, and assess their impact on mesangial cell function. |
| Shipped in | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | No |
| Mycoplasma Test | Negative |
| Bacteria Test | Negative |
| Yeast Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove old medium from adherent cells and wash with calcium- and magnesium-free PBS. For T25 flasks, use 3-5 ml of PBS and for T75 flasks, use 5-10 ml. 2. Then, completely cover the cells with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. 3. Allow cells to detach by incubating at room temperature for 8-10 minutes. 4. After incubation, cells were gently mixed with 10 ml of medium to resuspend and centrifuged at 300xg for 3 min. 5. Discard the supernatant, resuspend the cells in fresh medium, and transfer them to a new flask that already contains fresh medium. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | The ratio of 1:2 to 1:5 is recommended. |
| Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%;Temperature: 37℃ |
| Cryopreservation | Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
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